增殖性衰老间充质间质细胞的免疫表型

IF 0.4 Q4 MULTIDISCIPLINARY SCIENCES
A. Ratushnyy
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引用次数: 0

摘要

间充质基质细胞(MSCs)是成体组织中的一类祖细胞,参与了组织的生理更新和损伤诱导再生过程。间充质干细胞作为一种再生药物已被广泛研究。在这方面,应该考虑MSC亚群的组织特异性特征。间充质干细胞具有许多潜在的有益特性,这些特性可以随着年龄的增长而显著改变。细胞响应外部信号和调节其功能状态的能力通常归因于细胞膜上的受体库。本文研究衰老脂肪源性间充质干细胞(AD-MSCs)的表面标志物表达。长期培养导致繁殖性衰老。在AD-MSCs上CD29、CD44、CD54、CD73、CD90和HLA-ABC的表达增加。在实验条件下,CD105和CD51/61的表达变化不可靠。所揭示的效应不仅与更大的细胞尺寸或更高的自身荧光有关,而且与细胞表面单位面积上标记物数量的增加有关。检测到的变化可能是衰老间充质干细胞特性的许多改变的基础,包括迁移、粘附、免疫调节和血管生成活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Immunophenotype of Replicative Senescent Mesenchymal Stromal Cells
Mesenchymal stromal cells (MSCs), a population of progenitor cells in adult tissues, are involved in the processes of physiological tissue renewal and damage-induced regeneration. MSCs have been widely studied as regenerative medicine agents. In this regard, the tissue-specific features of MSC subpopulations should be taken into account. MSCs have many potentially beneficial properties that can alter significantly with age. The ability of a cell to respond to external signals and regulate its functional state is commonly attributed to the repertoire of receptors on the cell membrane. This article considers the surface marker expression of senescent adipose-derived MSCs (AD-MSCs). Replicative senescence was caused by long-term cultivation. An increase in the expression of CD29, CD44, CD54, CD73, CD90, and HLA-ABC on the AD-MSCs was shown. The expression of CD105 and CD51/61 did not change reliably under the experimental conditions. The revealed effects are related not only to the larger cell size or higher autofluorescence, but also to the increased number of markers per unit area of the cell surface. The detected changes may underlie a number of modifications in the properties of senescent MSCs, including migration, adhesion, and immunomodulatory and angiogenic activities.
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来源期刊
CiteScore
0.70
自引率
0.00%
发文量
0
审稿时长
17 weeks
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