揭示无毒氟离子液体水溶液对溶菌酶包封及稳定性的影响

Margarida L. Ferreira, N. S. Vieira, J. Araújo, A. B. Pereiro
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引用次数: 4

摘要

蛋白质是一种具有生物活性的化合物,在生物制药工业、食品科学和生物催化剂方面具有很高的应用潜力。然而,蛋白质在天然环境之外很难保持稳定性,这阻碍了它们的应用。氟化离子液体(FILs)是一类很有前途的表面活性离子液体(SAILs),具有两亲性和在水溶液中通过形成胶体体系自聚集的能力。本研究选择蛋白溶菌酶来推测FILs对其稳定性和活性的影响。然后,测定FILs的细胞毒性以评估其生物相容性,得出所选化合物忽略细胞毒性的结论。因此,利用紫外可见光谱法对FILs与溶菌酶的相互作用进行了推断,认为FILs对溶菌酶的包封作用是主要的相互作用。包封效率与FIL的浓度和负离子密切相关。最后,对溶菌酶的生物活性和热稳定性进行了评价,包封的溶菌酶保持了活性和热稳定性,表明FILs可以作为一种潜在的稳定剂用于蛋白质基递送系统。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Unveiling the Influence of Non-Toxic Fluorinated Ionic Liquids Aqueous Solutions in the Encapsulation and Stability of Lysozyme
Proteins are bioactive compounds with high potential to be applied in the biopharmaceutical industry, food science and as biocatalysts. However, protein stability is very difficult to maintain outside of the native environment, which hinders their applications. Fluorinated ionic liquids (FILs) are a promising family of surface-active ionic liquids (SAILs) that have an amphiphilic behavior and the ability to self-aggregate in aqueous solutions by the formation of colloidal systems. In this work, the protein lysozyme was selected to infer on the influence of FILs in its stability and activity. Then, the cytotoxicity of FILs was determined to evaluate their biocompatibility, concluding that the selected compounds have neglected cytotoxicity. Therefore, UV–visible spectroscopy was used to infer the FIL-lysozyme interactions, concluding that the predominant interaction is the encapsulation of the lysozyme by FILs. The encapsulation efficiency was also tested, which highly depends on the concentration and anion of FIL. Finally, the bioactivity and thermal stability of lysozyme were evaluated, and the encapsulated lysozyme keeps its activity and thermal stability, concluding that FILs can be a potential stabilizer to be used in protein-based delivery systems.
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