{"title":"梭形希兹克菌蛋白消化酶抑制肽的分离与鉴定","authors":"K. Suetsuna","doi":"10.2331/SUISAN.64.862","DOIUrl":null,"url":null,"abstract":"A peptidic fraction (SP-I) having inhibitory activity against angiotensin I-converting enzyme (ACE) was separated from the peptic digest of protein prepared from the brown alga Hizikia fusiformis by ion-exchange chromatographies and gel filtration. Oral administration of SP-I into spontaneously hypotensive rats at 200mg/kg resulted in a marked hypotensive effect. Further separation of SP-I by ODS HPLC afforded three active peptides, Gly-Lys-Tyr (IC50; 3.92μM), Ser-Val-Tyr (8.12μM), and Ser-Lys-Thr-Tyr (11.07μM).","PeriodicalId":9361,"journal":{"name":"Bulletin of the Japanese Society of Scientific Fisheries","volume":"3 1","pages":"862-866"},"PeriodicalIF":0.0000,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"36","resultStr":"{\"title\":\"Separation and identification of angiotensin I-converting enzyme inhibitory peptides from peptic digest of Hizikia fusiformis protein\",\"authors\":\"K. Suetsuna\",\"doi\":\"10.2331/SUISAN.64.862\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A peptidic fraction (SP-I) having inhibitory activity against angiotensin I-converting enzyme (ACE) was separated from the peptic digest of protein prepared from the brown alga Hizikia fusiformis by ion-exchange chromatographies and gel filtration. Oral administration of SP-I into spontaneously hypotensive rats at 200mg/kg resulted in a marked hypotensive effect. Further separation of SP-I by ODS HPLC afforded three active peptides, Gly-Lys-Tyr (IC50; 3.92μM), Ser-Val-Tyr (8.12μM), and Ser-Lys-Thr-Tyr (11.07μM).\",\"PeriodicalId\":9361,\"journal\":{\"name\":\"Bulletin of the Japanese Society of Scientific Fisheries\",\"volume\":\"3 1\",\"pages\":\"862-866\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1998-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"36\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bulletin of the Japanese Society of Scientific Fisheries\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2331/SUISAN.64.862\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bulletin of the Japanese Society of Scientific Fisheries","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2331/SUISAN.64.862","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Separation and identification of angiotensin I-converting enzyme inhibitory peptides from peptic digest of Hizikia fusiformis protein
A peptidic fraction (SP-I) having inhibitory activity against angiotensin I-converting enzyme (ACE) was separated from the peptic digest of protein prepared from the brown alga Hizikia fusiformis by ion-exchange chromatographies and gel filtration. Oral administration of SP-I into spontaneously hypotensive rats at 200mg/kg resulted in a marked hypotensive effect. Further separation of SP-I by ODS HPLC afforded three active peptides, Gly-Lys-Tyr (IC50; 3.92μM), Ser-Val-Tyr (8.12μM), and Ser-Lys-Thr-Tyr (11.07μM).
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