氨苄西林水解降解组氨酸的动力学分光光度法

Ivana Rasic-Misic, S. Tošić, Emilija T. Pecev-Marinković, D. Kostić, B. Arsić
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引用次数: 0

摘要

本研究的目的是建立一种动态分光光度法测定纯形式和膳食补充剂中l -组氨酸的微量含量。该方法是基于Ni(II)离子作为催化剂,在l -组氨酸存在下,在强碱性介质中降解氨苄西林的动力学。通过测量265 nm处吸光度随时间的变化来监测反应速率。同样的方法被用于研究不含组氨酸的反应速率。采用微分法的正切法处理动力学数据。啤酒吗?组氨酸浓度在1.24 μ g/ml ~ 11.63 μ g/ml范围内符合S定律,相对标准偏差为8.1% ~ 0.7%。根据3so0标准估计检出限为0.46 μ g/ml。研究了一些金属离子、阴离子和其他分子对反应速率的干扰作用,以评价方法的选择性。本方法用于膳食补充剂中组氨酸的定量测定。点假设检验证实本文方法与参考方法无显著性差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Kinetic–spectrophotometric approach to the ampicillin hydrolytic degradation applied for the histidine determination
The objective of this research was to develop a kinetic-spectrophotometric method for the determination of microquantities of L-histidine in pure form and dietary supplements. The method was based on the kinetics of ampicillin degradation by Ni(II) ion as a catalyst in the presence of L-histidine in a strongly alkaline medium. The rate of this reaction was monitored spectrophotometrically by measuring the increase in absorbance at 265 nm as a function of time. The same approach was used for the investigation of the reaction rate in the absence of histidine. A differential variant of the tangent method was used to process the kinetic data. Beer?s law was obeyed in the interval of histidine concentration from 1.24 ?g/ml to 11.63 ?g/ml with the relative standard deviation ranging from 8.1% to 0.7%. The detection limit of 0.46 ?g/ml was estimated based on the 3S0 criterion. The interference effects of some metal ions, anions, and other molecules on the reaction rate were studied to assess method selectivity. Herein described method was applied for the quantification of histidine in dietary supplements. The point hypothesis test confirmed that there was no significant difference between the proposed and the reference method.
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