{"title":"一个更好的开始","authors":"S. Batard, C. Laine","doi":"10.1002/IMIC.200890077","DOIUrl":null,"url":null,"abstract":"The success of in vitro fertilisation techniques such as intracytoplasmic sperm injection (ICSI) depends critically on selection of the most healthy sperm for injection into the oocyte. Intracytoplasmic morphologically selected sperm injection (IMSI) is a high magnification light microscopy imaging method that is being used increasingly to select sperm for ICSI. IMSI has been shown to increase pregnancy rates and reduce abortion rates compared to routine ICSI-IVF. This technical note describes IMSI using a Nikon inverted microscope equipped with Normarski DIC optics and digital imaging system to achieve magnifications in excess of 6000x.","PeriodicalId":100658,"journal":{"name":"Imaging & Microscopy","volume":"75 2 1","pages":"58-59"},"PeriodicalIF":0.0000,"publicationDate":"2008-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A Better Start in Life\",\"authors\":\"S. Batard, C. Laine\",\"doi\":\"10.1002/IMIC.200890077\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The success of in vitro fertilisation techniques such as intracytoplasmic sperm injection (ICSI) depends critically on selection of the most healthy sperm for injection into the oocyte. Intracytoplasmic morphologically selected sperm injection (IMSI) is a high magnification light microscopy imaging method that is being used increasingly to select sperm for ICSI. IMSI has been shown to increase pregnancy rates and reduce abortion rates compared to routine ICSI-IVF. This technical note describes IMSI using a Nikon inverted microscope equipped with Normarski DIC optics and digital imaging system to achieve magnifications in excess of 6000x.\",\"PeriodicalId\":100658,\"journal\":{\"name\":\"Imaging & Microscopy\",\"volume\":\"75 2 1\",\"pages\":\"58-59\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2008-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Imaging & Microscopy\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1002/IMIC.200890077\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Imaging & Microscopy","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/IMIC.200890077","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The success of in vitro fertilisation techniques such as intracytoplasmic sperm injection (ICSI) depends critically on selection of the most healthy sperm for injection into the oocyte. Intracytoplasmic morphologically selected sperm injection (IMSI) is a high magnification light microscopy imaging method that is being used increasingly to select sperm for ICSI. IMSI has been shown to increase pregnancy rates and reduce abortion rates compared to routine ICSI-IVF. This technical note describes IMSI using a Nikon inverted microscope equipped with Normarski DIC optics and digital imaging system to achieve magnifications in excess of 6000x.