一个更好的开始

S. Batard, C. Laine
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引用次数: 0

摘要

体外受精技术的成功,如胞浆内单精子注射(ICSI),关键取决于选择最健康的精子注射到卵母细胞。胞浆内形态学选择精子注射(IMSI)是一种高倍率光学显微镜成像方法,越来越多地用于选择用于ICSI的精子。与常规的ICSI-IVF相比,IMSI已被证明可以增加怀孕率并降低流产率。本技术说明描述了IMSI使用配备Normarski DIC光学和数字成像系统的尼康倒置显微镜,以实现超过6000倍的放大倍率。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A Better Start in Life
The success of in vitro fertilisation techniques such as intracytoplasmic sperm injection (ICSI) depends critically on selection of the most healthy sperm for injection into the oocyte. Intracytoplasmic morphologically selected sperm injection (IMSI) is a high magnification light microscopy imaging method that is being used increasingly to select sperm for ICSI. IMSI has been shown to increase pregnancy rates and reduce abortion rates compared to routine ICSI-IVF. This technical note describes IMSI using a Nikon inverted microscope equipped with Normarski DIC optics and digital imaging system to achieve magnifications in excess of 6000x.
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