含有基因工程大肠杆菌DH5细胞的人工细胞用于肾脏和肝脏衰竭的尿素和氨的去除

Proceedings of 17th International Conference of the Engineering in Medicine and Biology Society Pub Date : 1995-09-20 DOI:10.1109/IEMBS.1995.579913

S. Prakash, T. Chang

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引用次数: 0

摘要

描述了一种利用微囊化基因工程大肠杆菌DH5细胞去除尿素和氨的新方法。在体外，100 mg被海藻酸-聚赖氨酸-海藻酸盐(APA)膜包裹的细菌细胞，在其log相生长状态下，在20分钟内可降低87.89/spl plusmn/2.25%的血浆尿素，在30分钟内可降低99.99%。同样数量的包封细菌也能在30分钟内将氨从975.14/spl plusmn/70.15 /spl mu/M/L降低到81.151/spl plusmn/7.37 /spl mu/M/L。这种新方法明显比目前可用的尿素和氨去除方法更有效。

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Artificial cells containing genetically engineered E. coli DH5 cells for urea and ammonia removal in kidney and liver failure

Describes a novel method of urea and ammonia removal using microencapsulated genetically engineered E. coli DH5 cells. In vitro, 100 mg of bacterial cells encapsulated in alginate-polylysine-alginate (APA) membrane, in their log phase state of growth, can lower 87.89/spl plusmn/2.25% of the plasma urea within 20 minutes and 99.99% in 30 minutes. The same amount of encapsulated bacteria is also capable of lowering ammonia from 975.14/spl plusmn/70.15 /spl mu/M/L to 81.151/spl plusmn/7.37 /spl mu/M/L in 30 minutes. This new approach is significantly more efficient than the presently available methods of urea and ammonia removal.

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Proceedings of 17th International Conference of the Engineering in Medicine and Biology Society

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