Pengaruh Lama Autoclave Terhadap Kualitas Kimia Wheat Pollard Yang Berpotensi Sebagai Prebiotik

Penelitian bertujuan mengkaji pengaruh lama pemanasan wheat pollard dengan autoclave untuk mendapatkan monomer-monomer yang berpotensi sebagai prebiotik.  Penelitian menggunakan rancangan acak lengkap pola searah dengan 4 perlakuan dan 4 ulangan. Parameter yang diamati adalah kadar air, abu, lemak kasar, protein kasar, serat kasar dan bahan ekstrak tanpa nitrogen (BETN), acid detergent fibre (ADF), neutral detergent fibre (NDF), selulosa, hemiselulosa, lignin, gross energi, mannosa, arabinosa, glukosa, sukrosa, rafinosa, amilosa, amilum, amilopektin, resisten starch dan profil wheat pollard melalui scanning electron microscope (SEM).  Hasil penelitian menunjukkan bahwa terdapat pengaruh perlakuan (p<0.05) terhadap kadar air, abu, protein kasar, serat kasar, BETN, NDF, hemiselulosa, lignin, selulosa, gros energi, rafinosa, glukosa, arabinosa, sukrosa, amilosa, amilum, amilopektin, resistan starch namun pada lemak kasar, ADF dan manosa tidak berpengaruh nyata (p>0.05). Indikator wheat pollard sebagai prebiotik terlihat dari peningkatan kadar rafinosa, arabinosa dan resistan starch berturut-turut sebesar 0.72% menjadi 3.95%; 0.51% menjadi 1.04%; 0.51% menjadi 1.04% dan 5.28% menjadi 14.15%.  Kesimpulan penelitian adalah wheat pollard yang diautoclave selama 15 menit memberikan komposisi terbaik sebagai prebiotik.The objective of this study was to examine the long-term effect of warming wheat pollard by autoclave to obtain potentially prebiotic monomers. The study used a completely randomized design pattern in the same direction with 4 treatments and 4 replications. The parameters observed were water content, ash, crude fat, crude protein, crude fiber, extract without nitrogen, acid detergent fiber (ADF), neutral detergent fiber (NDF), cellulose, hemicellulose, lignin, gross energy, mannosa, arabinose, glucose, sucrose, raffinose, amylose, starch, amylopectin, resistance starch and wheat pollard profile through scanning electron microscope (SEM). The results showed that there was a treatment effect (p <0.05) on moisture, ash, crude protein, crude fiber, BETN, NDF, hemicellulose, lignin, cellulose, gros energy, raffinose, glucose, arabinose, sucrose, amylose, starch, amylopectin , resistance starch but in crude fat, ADF and mannose did not have a significant effect (p>0.05). The indicator of wheat pollard as a prebiotic is seen from an increase in raffinose, arabinose and resistance starch levels of 0.72% to 3.95%; 0.51% to 1.04%; 0.51% to 1.04% and 5.28% to 14.15%. The conclusion of the study was that wheat pollard which was autoclaved for 15 minutes gave the best composition as a prebiotic.