大鼠睾丸原癌基因c-src的克隆

Otor Al-Khalilt, B. Duke, S. Zeltwanger, D. Eaton, B. Spier, J. Stockand
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引用次数: 6

摘要

利用高强度聚合酶链反应从大鼠睾丸中克隆出癌基因v-src的细胞同源物,即原癌基因c-src。大鼠c-src cDNA分别与鸡和小鼠、劳斯肉瘤病毒c-src和v-src同源。大鼠c-Src蛋白与人和小鼠c-Src蛋白同源性均达98%。有趣的是,与小鼠的蛋白质相比,大鼠的Src含有一个额外的氨基酸。正如预期的那样,大鼠睾丸Src缺乏与人类和小鼠中发现的神经元Src共同的6个额外残基。报告非神经元大鼠c-src的cDNA序列,将有助于大鼠组织作为人类疾病模型的细胞生长和转化实验。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cloning of the Proto-oncogene c-src from Rat Testis
The cellular homolog of the oncogene v-src, the proto-oncogene c-src, was cloned from rat testis using a high stringency polymerase chain reaction. Rat c-src cDNA shared identity with chicken and mouse, and Rous sarcoma virus c-src and v-src, respectively. Rat c-Src protein was 98% homologous to both human and mouse c-Src. Interestingly, rat Src contained one extra amino acid compared to the mouse protein. As expected, the rat testis Src lacked the six extra residues common to the neuronal Src identified in human and mouse. Reporting of the cDNA sequence for non-neuronal, rat c-src should facilitate experimentation into cell growth and transformation using rat tissues as models of human disease.
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