Sang-Su Kim, Jae‐Eup Park, Ye-Jin Kim, Yong-Hwan Lee, In‐Yong Lee, Jeongran Lee, Byeng-Chul Moon, Y. Ihm
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引用次数: 1
摘要
研究了国产水稻品种对4-羟基苯基丙酮酸双加氧酶(4-HPPD)抑制除草剂的植物毒性及除草剂反应敏感基因的特征。5个水稻品种分别为4粳型(尚珠别、三百别、山都真美和金荣)和1籼型(IR8),在育苗盘上种植2 ~ 3叶期,然后移栽到塑料钵中。我们在移植后10天试验了标准剂量(14 g a.i 10 a−1)和双倍剂量(28 g a.i 10 a−1)的苯并双环龙治疗,以研究植物毒性。粳稻型尚菊玉未对水稻造成伤害,而籼稻型IR8则表现出4-5(标准)和5-6(双倍)的植物毒性。虽然是日本式,但山都真美和金荣分别是3-4级和4-5级。抗性基因羟基苯基丙酮酸双加氧酶抑制剂敏感基因1号(HIS1)从国内5个水稻品种(尚珠别、三百别、山都真美、金荣和IR8)中分离得到。结果表明,5个水稻品种均含有HIS1基因。并从HIS1序列中鉴定出不同水稻品种间存在的一些基因变异。
Characteristics of Sensitive HIS1 Genes to the 4-HPPD Inhibiting Rice Herbicides Isolated from Several Rice Cultivars
This study was conducted to determine phytotoxicity of domestic rice varieties and characteristic of sensitive gene involved in herbicide reaction of 4-HPPD (4-hydroxy phenylpyruvate dioxygenase) inhibiting herbicides. The five rice varieties were grown for 2 to 3 leaf stage on seedling trays and then transplanted into plastic pot: 4-Japonica type (Sangjubyeo, Sambaekbyeo, Sanduljinmi and Kumyoung) varieties and 1-Indica type variety (IR8). We trialled standard (14 g a.i. 10 a −1 ) and double fold (28 g a.i. 10 a −1 ) dose of benzobicyclon treatment at 10 days after transplanting in order to investigate phytoxicity. The Japonica-type Sangjubyeo showed no rice injury but Indica-type IR8 show 4-5 (standard) and 5-6 (double fold) phytoxicity levels. In spite of Japonica-type, Sanduljinmi and kumyoung showed 3-4 and 4-5 levels. Target resistant gene, Hydroxyphenylpyruvate dioxygenase inhibitor sensitive gene No.1 (HIS1) have been isolated from five domestic rice cultivars (Sangjubyeo, Sambaekbyeo, Sanduljinmi, Kumyoung and IR8). Results of the sequence through PCR, all five tested rice cultivars had HIS1 gene regardless to rice cultivars. And the difference between rice varieties from sequence of HIS1 were identified some variation in genes.