从tnf诱导的信号传导到NADPH氧化酶活性:研究参与调节细胞死亡方式的蛋白质复合物的方法

Maria Ladik, Hana Valenta, M. Erard, P. Vandenabeele, Franck B. Riquet
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引用次数: 0

摘要

分子复合物的形成是细胞内信号通路的一个关键特征,它控制着特定细胞过程的启动和执行。肿瘤坏死因子(Tumor Necrosis Factor, TNF)和活性氧(Reactive Oxygen Species, ROS)作为信号分子,参与细胞生存与死亡之间的命运平衡。作为细胞信号传导的主要调控因子,它们也在控制组织稳态、先天免疫和炎症的各种细胞过程中发挥重要作用。有趣的是,TNF和ROS是相互联系的,并通过分子信号复合物参与调节彼此的产生。这种关系需要在调节细胞死亡(RCD)模式的背景下详细审查tnf诱导和ros产生的分子复合物。在这里,我们概述了用于研究TNF-和关于ROS的NADPH氧化酶相关分子复合物的生物技术方法,重点是不同的受调节的细胞死亡方式。本系统综述强调了细胞死亡领域如何受益于生化和活细胞荧光成像方法。这些知识和已建立的工作流程具有高度的通用性,可以更广泛地用于任何蛋白质复合物的研究,并且非常适合解决信号动力学中的新挑战。这将有助于理解分子信号复合物作为被组织成信号平台的集合体,最有可能是信号动力学整合到细胞命运调节的关键位点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
From TNF-induced signaling to NADPH oxidase enzyme activity: Methods to investigate protein complexes involved in regulated cell death modalities
The formation of molecular complexes is a key feature of intracellular signaling pathways which governs to the initiation and execution of dedicated cellular processes. Tumor Necrosis Factor (TNF) and Reactive Oxygen Species (ROS) function as signaling molecules and are both involved in balancing cell fate decision between cell survival or cell demise. As master regulators of cell signaling, they are also instrumental in controlling various cellular processes towards tissue homeostasis, innate immunity and inflammation. Interestingly, TNF and ROS are interlinked and involved in regulating each other’s production via the engagement of molecular signaling complexes. This relationship calls for detailed reviewing of both TNF-induced and ROS-producing molecular complexes in the context of regulated cell death (RCD) modalities. Here, we outline biotechnological approaches that were used to investigate the TNF- and, concerning ROS, the NADPH oxidase-related molecular complexes with an emphasis on different regulated cell death modalities. This systematic review highlights how the cell death field has benefited from both biochemical and live-cell fluorescence imaging approaches. This knowledge and established workflows are highly generalizable, can be of a broader use for any protein-complex studies, and well suited for addressing new challenges in signaling dynamics. These will help understand molecular signaling complexes as ensembles organized into signaling platforms, most likely the key sites of signaling dynamics integration toward cell fate regulation.
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