SARS-CoV-2传染性和全身并发症的细胞培养模型

Q4 Pharmacology, Toxicology and Pharmaceutics

Pharmaceutical Sciences Asia Pub Date : 2022-01-01 DOI:10.29090/psa.2022.04.22.058

Khanit Sa-ngiamsuntorn, Piyanoot Thongsri, Yongyut Pewkliang, A. Wongkajornsilp

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引用次数: 0

摘要

自2020年3月以来，世卫组织宣布COVID-19为大流行。疫苗接种计划已在世界范围内实施。特异性抗病毒药物如remdesivir、molnupiravir和基于利托那韦的nirmatrelvir对SARS-CoV-2感染有效。然而，由于病毒突变导致疫苗耐药性和快速传播，新的SARS-CoV-2变体已经增加。在一些康复病例中，COVID-19的长期并发症已危及生命。为了克服病毒的适应性，细胞培养模型对于理解SARS-CoV-2感染、病理生理、并发症和药物靶点改变至关重要。采用经典二维培养细胞进行病毒繁殖和高通量筛选。现代3D培养再现了组织生理学的关键细胞和分子事件。本文对上述应用的细胞系、三维培养、类器官和相关模型进行了综述。

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Cell culture models for SARS-CoV-2 infectivity and systemic complications

COVID-19 was declared by WHO as a pandemic since March 2020. The vaccination program has been implemented worldwide. Specific antiviral drugs such as remdesivir, molnupiravir and ritonavir-based nirmatrelvir were effective against SARS-CoV-2 infection. However, the new SARS-CoV-2 variants have been elevated due to viral mutation causing vaccine resistance and rapid spreading. Long-term COVID-19 complications have been lifethreatening in some recovery cases. To overcome viral adaptation, cell culture model is essential to comprehend SARS-CoV-2 infection, pathophysiology, complications, and drug target alterations. The classical 2D culture cell was frequency used for viral propagation and high-throughput screening. Modern 3D culture has recapitulated key cellular and molecular events of tissue physiology. Here, we reviewed the cell lines, 3D culture, organoid and relevant models for the aforementioned applications.

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来源期刊

Pharmaceutical Sciences Asia Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)

CiteScore

0.90

自引率

0.00%

发文量

期刊介绍： The Pharmaceutical Sciences Asia (PSA) journal is a double-blinded peer-reviewed journal in English published quarterly, by the Faculty of Pharmacy, Mahidol University, Thailand. The PSA journal is formerly known as Mahidol University Journal of Pharmaceutical Sciences and committed to the timely publication of innovative articles and reviews. This journal is available in both printed and electronic formats. The PSA journal aims at establishing a publishing house that is open to all. It aims to disseminate knowledge; provide a learned reference in the field; and establish channels of communication between academic and research expert, policy makers and executives in industry and investment institutions. The journal publishes research articles, review articles, and scientific commentaries on all aspects of the pharmaceutical sciences and multidisciplinary field in health professions and medicine. More specifically, the journal publishes research on all areas of pharmaceutical sciences and related disciplines: Clinical Pharmacy Drug Synthesis and Discovery Targeted-Drug Delivery Pharmaceutics Biopharmaceutical Sciences Phytopharmaceutical Sciences Pharmacology and Toxicology Pharmaceutical Chemistry Nutraceuticals and Functional Foods Natural Products Social, Economic, and Administrative Pharmacy Clinical Drug Evaluation and Drug Policy Making Antimicrobials, Resistance and Infection Control Pharmacokinetics and Pharmacodynamics.