The effect of L-arginine therapy on hepatocyte cells damage in mice ( Mus musculus ) model of preeclampsia

Preeclampsia is one of the main contributors on morbidity and mortality of mother and fetus. Imbalance of body antioxidants and oxidants has a role on endothelial destruction causing endothelial dysfunction which leads to preeclampsia. L-arginine administration is indicated to be able to fix the remodelling process of hepatocytes which was damaged on preeclampsia. The aim of this study was to investigate the effect of L-arginine on hepatocyte cells damage on mouse ( Mus musculus ) model of preeclampsia. Experimental study was conducted in the Experimental Animal Warehouse and Biomedical Laboratory, Faculty of Veterinary Medicine, Airlangga University, Surabaya. A total of 30 pregnant mice were divided into three groups with 10 mice in each group i.e. normal, preeclampsia model and preeclampsia model with L-arginine therapy pregnant mice. Preparates were taken from dissected mice liver, with prior paraffin block and were then stained with hematoxylin-eosin. Results were analyzed semi quantitatively with Manja Roenigk hepar histology scoring method. Data were analyzed using Kruskal-Wallis and Man-Whitney test. Mean of hepar histopathology score of preeclampsia model with L-arginine therapy (40.80±1.17/µm 2 ) was significantly lower compared with preeclampsia model pregnant mice (58.80±4.23/µm 2 ) (p<0.05), however it was significantly higher compared with normal pregnant mice (18.40±2.41/µm 2 ) (p<0.05). It was indicated that the L-arginine therapy on preeclampsia mouse model can reduce the score of hepar histopathology. In conclusion, L-arginine administration can reduce the hepatocyte cells damage of mouse due to preeclampsia.