{"title":"细菌、植物和动物来源的尿囊酶1 .纯化和酶的性质","authors":"G.D. Vogels, F. Trijbels, A. Uffink","doi":"10.1016/0926-6593(66)90040-3","DOIUrl":null,"url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. Allantoinases (allantoin amidohydrolase, EC 3.5.2.5) from <em>Streptococcus allantoicus, Arthrobacter allantoicus, Escherichia coli, Pseudomonas fluorescens, Pseudomonas acidovorans</em>, frog liver, goldfish liver, <em>Phaseolus hysterinus</em> and <em>Glycine hispida</em> were studied.</p></span></li><li><span>2.</span><span><p>2. The enzymes were purified 2.5- to 37-fold by (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub> precipitation and DEAE-cellulose chromatography.</p></span></li><li><span>3.</span><span><p>3. The pH optimum curve, Michaelis constant, activation energy, stability and specificity were determined and compared.</p></span></li><li><span>4.</span><span><p>4. The enzymes from <em>S. allantoicus, A. allantoicus</em> and <em>E. coli</em> were aspecific; the others degraded (+)-allantoin 4–22 times faster than (−)-allantoin.</p></span></li><li><span>5.</span><span><p>5. The allantoinases from <em>Ph. hysterinus</em> and <em>G. hispida</em> were activated by acid pretreatment below pH 5.4.</p></span></li></ul></div>","PeriodicalId":100160,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation","volume":"122 3","pages":"Pages 482-496"},"PeriodicalIF":0.0000,"publicationDate":"1966-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6593(66)90040-3","citationCount":"46","resultStr":"{\"title\":\"Allantoinases from bacterial, plant and animal sources I. Purification and enzymic properties\",\"authors\":\"G.D. Vogels, F. Trijbels, A. Uffink\",\"doi\":\"10.1016/0926-6593(66)90040-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p></p><ul><li><span>1.</span><span><p>1. Allantoinases (allantoin amidohydrolase, EC 3.5.2.5) from <em>Streptococcus allantoicus, Arthrobacter allantoicus, Escherichia coli, Pseudomonas fluorescens, Pseudomonas acidovorans</em>, frog liver, goldfish liver, <em>Phaseolus hysterinus</em> and <em>Glycine hispida</em> were studied.</p></span></li><li><span>2.</span><span><p>2. The enzymes were purified 2.5- to 37-fold by (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub> precipitation and DEAE-cellulose chromatography.</p></span></li><li><span>3.</span><span><p>3. The pH optimum curve, Michaelis constant, activation energy, stability and specificity were determined and compared.</p></span></li><li><span>4.</span><span><p>4. The enzymes from <em>S. allantoicus, A. allantoicus</em> and <em>E. coli</em> were aspecific; the others degraded (+)-allantoin 4–22 times faster than (−)-allantoin.</p></span></li><li><span>5.</span><span><p>5. The allantoinases from <em>Ph. hysterinus</em> and <em>G. hispida</em> were activated by acid pretreatment below pH 5.4.</p></span></li></ul></div>\",\"PeriodicalId\":100160,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation\",\"volume\":\"122 3\",\"pages\":\"Pages 482-496\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1966-09-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0926-6593(66)90040-3\",\"citationCount\":\"46\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0926659366900403\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926659366900403","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}