以蔗糖为唯一碳源的澳大利亚偶氮单胞菌制备聚羟基丁酸生物聚合物及其特性研究

Salman Ahmad, Abuzer Amir, Md. Zafaryab, K. Osama, Soban Ahmad Faridi, M. H. Siddiqui, M. MoshahidARizvi, Md. Asad Khan
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引用次数: 6

摘要

选择了一株澳大利亚偶氮单胞菌DSM 1124,该菌株在细胞内积累了聚β-羟基丁酸颗粒,并对各种方法在PHB提取中的优缺点进行了比较。我们的研究选择了氯仿-次氯酸钠法。这是提取澳大利亚偶氮单胞菌DSM 1124中PHB的方法之一。聚羟基丁酸酯是一种可生物降解和生物相容性好的热塑性塑料,在医药、食品包装和组织工程材料中有许多有趣的应用。它不会像石油基塑料那样在环境中产生任何毒素或残留物。本研究着重研究了澳大利亚偶氮单胞菌以蔗糖为唯一碳源提高PHB的产量,并对培养基中生物量和蔗糖含量进行了估算。采用优化的培养基配方,在7 L的生物反应器中进行间歇培养,最大生物量为1.71 g/L, PHB浓度为2.67 g/L。通过生长动力学研究、紫外分光光度计和傅里叶变换红外光谱(FTIR)对PHB进行了表征。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Production and Characterization of Polyhydroxybutryrate Biopolymer from Azohydromonas australica Using Sucrose as a Sole Carbon Source
A bacterial strain Azohydromonas australica DSM 1124 has been chosen, which is accumulated intracellular poly- β-hydroxybutyrate particles, each method which has been merit and demerit for employed in PHB extraction. Our study, we have selected chloroform-sodium hypochlorite method. It is one of the methods for extracting PHB forms Azohydromonas australica DSM 1124. Polyhydroxybutyrate is a biodegradable and biocompatible thermoplastic with many interesting applications in medicine, food packaging and tissue engineering materials. That does not produce any toxins or residues in the environment like petroleum based plastics. The present study was emphasized on enhanced production of PHB by Azohydromonas australica using sucrose as a sole carbon source and estimation of biomass and sucrose content in the media.The batch kinetics analysis of Azohydromonas australica was done an interval of 3 h. Batch cultivation with optimized media recipe in a 7 L bioreactor exhibited a maximum biomass 1.71 g/L and PHB concentration 2.67 g/L for A. australica. The characterization of PHB was done by growth kinetics studies, UV-Spectrophotometer and Fourier transforms infrared spectroscopy (FTIR).
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