外源一氧化氮对NaCl处理鹰嘴豆植株氧化代谢的改善作用

S. Sheokand, V. Bhankar, V. Sawhney
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引用次数: 73

摘要

灌溉土壤中盐分的积累是限制南亚产量的主要因素之一。研究了外源一氧化氮(NO)作为硝普钠(sodium nitroprusside)的补充是否对鹰嘴豆叶片NaCl诱导的氧化损伤有改善作用。在50日龄鹰嘴豆植株上分别施用250 mM NaCl和0.2、1 mM SNP,处理2、4、6 d。盐胁迫对相对膜损伤、脂质过氧化水平、相对含水量(RWC)和H2O2含量均有不利影响。效果与时间有关。SNP处理可改善短期盐胁迫2 d对相对膜损伤的毒性作用,4 d和6 d的盐胁迫可部分改善相对膜损伤。脂质过氧化水平、H2O2含量和RWC对SNP有部分改善作用。盐胁迫通过提高SOD、POX、APX和DHAR活性激活抗氧化系统。盐胁迫下GR活性变化不明显,CAT活性下降。盐胁迫下,SNP处理对水稻抗氧化酶SOD、CAT、APX、GR和DHAR均有显著的正向影响。NaCl处理导致GSH/GSSG和ASC/DHA比值下降。SNP处理增加了这两种代谢物的还原形式,从而提高了GSH/GSSG和ASC/DHA的比例。本研究表明,外源施用NO可保护鹰嘴豆叶片免受NaCl诱导的氧化胁迫。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Ameliorative effect of exogenous nitric oxide on oxidative metabolism in NaCl treated chickpea plants
Accumulation of salts in irrigated soils is one of the primary factors limiting yield in South Asia. We investigated whether exogenous nitric oxide (NO) supplementation as sodium nitroprusside has any ameliorating effect against NaCl induced oxidative damage in chickpea leaves. NaCl treatment (250 mM) alone and in combination with two concentrations of SNP (0.2 and 1 mM) were given to 50 days old chickpea plants for 2, 4 and 6 days. Salt stress adversely affected the relative membrane injury, lipid peroxidation levels, relative water content (RWC) and H2O2 content. The effect was time dependent. SNP treatments could ameliorate the toxic effect of short term salt stress of 2 days on relative membrane injury and partial amelioration was observed with 4 and 6 day stress treatment. A partial ameliorative effect of SNP was observed with lipid peroxidation levels, H2O2 content and RWC. Salt stress activated the antioxidant system by increasing the activities of SOD, POX, APX and DHAR. However no obvious change was observed in GR activity and CAT activity decreased under salt stress. Both the SNP treatments had a positive effect on antioxidant enzymes SOD, CAT, APX, GR and DHAR under salt stress. NaCl treatment resulted in a decline in the GSH/GSSG and ASC/DHA ratio. SNP treatments increased the reduced form of both the metabolites thus elevating the ratio of GSH/GSSG and ASC/DHA. This study concludes that exogenous application of NO protects chickpea leaves from NaCl induced oxidative stress.
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