{"title":"产自青霉菌的青霉素G酰化酶的生产、优化、纯化、抑菌性能和染料脱色性能测试。","authors":"Balaji Mahendiran","doi":"10.9790/3008-0735765","DOIUrl":null,"url":null,"abstract":"The enzyme Penicillin G Acylase (PGA) has been produced from Penicillium chrysogenum obtained from spoiled lemon sample. The physico-chemical parameters like carbon source, pH, temperature and media were optimized for higher production of enzyme. The enzyme was purified by ammonium sulphate precipitation followed by Dialysis and Ion-Exchange Chromatography and confirmed by TLC. The purified enzyme was tested for anti-microbial and dye-decolorizing properties. The enzyme showed a clear zone of inhibition when tested against few bacterial pathogens. Then the immobilized enzyme was tested for dye-decolourization activity against Congo red, which showed a very low activity as compared to that of Penicillium chrysogenum.","PeriodicalId":11924,"journal":{"name":"European Journal of Biotechnology and Bioscience","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2013-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Production, Optimization, Purification, Testing Anti-Microbial and Dye Decolorizing Properties of Penicillin G Acylase from Penicillium chrysogenum.\",\"authors\":\"Balaji Mahendiran\",\"doi\":\"10.9790/3008-0735765\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The enzyme Penicillin G Acylase (PGA) has been produced from Penicillium chrysogenum obtained from spoiled lemon sample. The physico-chemical parameters like carbon source, pH, temperature and media were optimized for higher production of enzyme. The enzyme was purified by ammonium sulphate precipitation followed by Dialysis and Ion-Exchange Chromatography and confirmed by TLC. The purified enzyme was tested for anti-microbial and dye-decolorizing properties. The enzyme showed a clear zone of inhibition when tested against few bacterial pathogens. Then the immobilized enzyme was tested for dye-decolourization activity against Congo red, which showed a very low activity as compared to that of Penicillium chrysogenum.\",\"PeriodicalId\":11924,\"journal\":{\"name\":\"European Journal of Biotechnology and Bioscience\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2013-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"European Journal of Biotechnology and Bioscience\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.9790/3008-0735765\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"European Journal of Biotechnology and Bioscience","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.9790/3008-0735765","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Production, Optimization, Purification, Testing Anti-Microbial and Dye Decolorizing Properties of Penicillin G Acylase from Penicillium chrysogenum.
The enzyme Penicillin G Acylase (PGA) has been produced from Penicillium chrysogenum obtained from spoiled lemon sample. The physico-chemical parameters like carbon source, pH, temperature and media were optimized for higher production of enzyme. The enzyme was purified by ammonium sulphate precipitation followed by Dialysis and Ion-Exchange Chromatography and confirmed by TLC. The purified enzyme was tested for anti-microbial and dye-decolorizing properties. The enzyme showed a clear zone of inhibition when tested against few bacterial pathogens. Then the immobilized enzyme was tested for dye-decolourization activity against Congo red, which showed a very low activity as compared to that of Penicillium chrysogenum.
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