{"title":"印度不同农业气候区采集的芸苔交替菌的致病性和分子变异","authors":"Maneesh Kumar Singh, H. Singh","doi":"10.15740/has/ijpp/12.2/176-182","DOIUrl":null,"url":null,"abstract":"The Alternaria blight is one of the most destructive fungal disease of Indian mustard causes severe damage to the crop. Ten isolates of A. brassicae were collected from various agro-climatic location of India viz., Uttar Pradesh (Ab 1 ), Madhya Pradesh (Ab 2 ), Uttarakhand (Ab 3 ), Bihar (Ab 4 ), Jharkhand (Ab 5 ), West Bengal (Ab 6 ), Haryana (Ab 7 ), Rajasthan (Ab 8 ), Chhattisgarh (Ab 9 ) and Gujarat (Ab 10 ) and characterized for pathogenic and molecular variations. All the isolates showed high level of variability. The incubation period of the isolates was recorded on B. juncea 3 to 4 days, B. carinata 6.17 to 6.83 days, B. napus 5.17 to 6.00 days, B. nigra 4.17 to 5.17 days and in B. campestris it was ranged from 3.17 to 4.00 days. The results revealed that the maximum PDI was noted on Brassica juncea followed by B. campestris var yellow sarson, B. nigra , B. napus and B. carinata . Based on PDI ten isolates could be classified into three groups in which group one consist of isolates Ab 3 , Ab 6 , Ab 7 and Ab 5 . Isolates Ab 8 , Ab 2 and Ab 4 fall in second group; while group three include isolates Ab 1 , Ab 9 and Ab 10 . The dendrogram analysis identified two major clusters with 82 per cent similarity. One cluster (group I) comprised of 3 isolates (Ab 1 , Ab 10 and Ab 2 ). Whereas, another cluster (group II) comprised of Ab 3 , Ab 6 , Ab 7 , Ab 5 , Ab 4 , Ab 8 and Ab 9 at 86 per cent similarity. The three isolates (Ab 3 , Ab 6 and Ab 7 ) of group II showed 100 per cent similarity based on molecular basis.","PeriodicalId":14349,"journal":{"name":"International Journal of Plant Protection","volume":"16 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Pathogenic and molecular variability among Brassica isolates of Alternaria brassicae collected from different agro-climatic regions of India\",\"authors\":\"Maneesh Kumar Singh, H. Singh\",\"doi\":\"10.15740/has/ijpp/12.2/176-182\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The Alternaria blight is one of the most destructive fungal disease of Indian mustard causes severe damage to the crop. Ten isolates of A. brassicae were collected from various agro-climatic location of India viz., Uttar Pradesh (Ab 1 ), Madhya Pradesh (Ab 2 ), Uttarakhand (Ab 3 ), Bihar (Ab 4 ), Jharkhand (Ab 5 ), West Bengal (Ab 6 ), Haryana (Ab 7 ), Rajasthan (Ab 8 ), Chhattisgarh (Ab 9 ) and Gujarat (Ab 10 ) and characterized for pathogenic and molecular variations. All the isolates showed high level of variability. The incubation period of the isolates was recorded on B. juncea 3 to 4 days, B. carinata 6.17 to 6.83 days, B. napus 5.17 to 6.00 days, B. nigra 4.17 to 5.17 days and in B. campestris it was ranged from 3.17 to 4.00 days. The results revealed that the maximum PDI was noted on Brassica juncea followed by B. campestris var yellow sarson, B. nigra , B. napus and B. carinata . Based on PDI ten isolates could be classified into three groups in which group one consist of isolates Ab 3 , Ab 6 , Ab 7 and Ab 5 . Isolates Ab 8 , Ab 2 and Ab 4 fall in second group; while group three include isolates Ab 1 , Ab 9 and Ab 10 . The dendrogram analysis identified two major clusters with 82 per cent similarity. One cluster (group I) comprised of 3 isolates (Ab 1 , Ab 10 and Ab 2 ). Whereas, another cluster (group II) comprised of Ab 3 , Ab 6 , Ab 7 , Ab 5 , Ab 4 , Ab 8 and Ab 9 at 86 per cent similarity. The three isolates (Ab 3 , Ab 6 and Ab 7 ) of group II showed 100 per cent similarity based on molecular basis.\",\"PeriodicalId\":14349,\"journal\":{\"name\":\"International Journal of Plant Protection\",\"volume\":\"16 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Plant Protection\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.15740/has/ijpp/12.2/176-182\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Plant Protection","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15740/has/ijpp/12.2/176-182","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
白疫病是印度芥菜最具破坏性的真菌病害之一,对作物造成严重危害。从印度北方邦(Ab 1)、中央邦(Ab 2)、北阿坎德邦(Ab 3)、比哈尔邦(Ab 4)、贾坎德邦(Ab 5)、西孟加拉邦(Ab 6)、哈里亚纳邦(Ab 7)、拉贾斯坦邦(Ab 8)、恰蒂斯加尔邦(Ab 9)和古吉拉特邦(Ab 10)等不同农业气候区采集了10株芸苔科芸苔科菌株,并对其致病性和分子变异进行了鉴定。所有分离株均表现出高度变异性。各菌株的潜伏期分别为:juncea 3 ~ 4 d, carinata 6.17 ~ 6.83 d, napus 5.17 ~ 6.00 d, nigra 4.17 ~ 5.17 d, campestris 3.17 ~ 4.00 d。结果表明,甘蓝型油菜(Brassica juncea)的PDI最高,其次为油菜、黄芥、黑芥、甘蓝型油菜和黑芥。根据PDI, 10株分离株可分为3个类群,其中类群1由分离株ab3、ab6、ab7和ab5组成。分离物ab8、ab2和ab4属于第二组;第3组包括ab1、ab9和ab10。树形图分析确定了两个主要的集群具有82%的相似性。一个簇(组I)由3个分离株(Ab 1、Ab 10和Ab 2)组成。然而,另一个簇(组II)由Ab 3, Ab 6, Ab 7, Ab 5, Ab 4, Ab 8和Ab 9在86%的相似性。ⅱ群的3株分离物(Ab 3、Ab 6和Ab 7)在分子基础上具有100%的相似性。
Pathogenic and molecular variability among Brassica isolates of Alternaria brassicae collected from different agro-climatic regions of India
The Alternaria blight is one of the most destructive fungal disease of Indian mustard causes severe damage to the crop. Ten isolates of A. brassicae were collected from various agro-climatic location of India viz., Uttar Pradesh (Ab 1 ), Madhya Pradesh (Ab 2 ), Uttarakhand (Ab 3 ), Bihar (Ab 4 ), Jharkhand (Ab 5 ), West Bengal (Ab 6 ), Haryana (Ab 7 ), Rajasthan (Ab 8 ), Chhattisgarh (Ab 9 ) and Gujarat (Ab 10 ) and characterized for pathogenic and molecular variations. All the isolates showed high level of variability. The incubation period of the isolates was recorded on B. juncea 3 to 4 days, B. carinata 6.17 to 6.83 days, B. napus 5.17 to 6.00 days, B. nigra 4.17 to 5.17 days and in B. campestris it was ranged from 3.17 to 4.00 days. The results revealed that the maximum PDI was noted on Brassica juncea followed by B. campestris var yellow sarson, B. nigra , B. napus and B. carinata . Based on PDI ten isolates could be classified into three groups in which group one consist of isolates Ab 3 , Ab 6 , Ab 7 and Ab 5 . Isolates Ab 8 , Ab 2 and Ab 4 fall in second group; while group three include isolates Ab 1 , Ab 9 and Ab 10 . The dendrogram analysis identified two major clusters with 82 per cent similarity. One cluster (group I) comprised of 3 isolates (Ab 1 , Ab 10 and Ab 2 ). Whereas, another cluster (group II) comprised of Ab 3 , Ab 6 , Ab 7 , Ab 5 , Ab 4 , Ab 8 and Ab 9 at 86 per cent similarity. The three isolates (Ab 3 , Ab 6 and Ab 7 ) of group II showed 100 per cent similarity based on molecular basis.