输卵管内给予一氧化氮合酶阻滞剂刺激黄体酮和催产素分泌,延长牛黄体的寿命。

Jerzy J. Jaroszewski, William Hansel
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引用次数: 31

摘要

为研究一氧化氮(NO)对牛黄体(CL)分泌功能的影响,采用两组(每组4头)处理:第1组(第11、12天)饲喂一氧化氮合成酶(NOS)抑制剂诺美加-硝基- l -精氨酸甲酯(L-NAME),第2组(第17、18天)饲喂L-NAME。所有治疗均采用腔内微透析系统(MDS)。在指定的天数内连续4小时输注药物,治疗前后各设4小时对照期。每隔半小时采集灌注液和颈静脉血样。分析灌注液样品中黄体酮(P4)、催产素(OT)、前列腺素f2 α (pgf2 α)和白三烯C4 (LTC4)的含量;分析颈静脉血浆样本的P4、OT和LH。在第11天或第12天灌注L-NAME持续增加灌注液中的P4浓度,但对CL的寿命没有影响。在第17-18天灌注L-NAME也提高了灌注液中的P4水平,此外,在周期的第25天,4只处理动物中的3只血浆中的P4水平保持不变。在黄体中期和晚期,L-NAME灌注也增加了OT伴随P4进入灌注液的释放。在大多数情况下,在灌注期间收集的颈静脉血浆样本中LH, OT和P4的浓度不受治疗的影响。L-NAME灌注引起灌注液PGF2alpha和LTC4在第17天和第18天,以及第11天和第12天LTC4的小幅但显著(P < 0.05)升高。这些数据表明,NO在牛CL的回归中起直接的解黄作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Intraluteal administration of a nitric oxide synthase blocker stimulates progesterone and oxytocin secretion and prolongs the life span of the bovine corpus luteum.
To test the role of nitric oxide (NO) in secretory functions of bovine corpora lutea (CL), two groups of four Holstein heifers each were treated as follows: Group 1, Nomega-Nitro-L-Arginine Methyl Ester (L-NAME), an inhibitor of nitric oxide synthase (NOS), on Day 11 or 12 of the cycle and Group 2, L-NAME on Days 17 and 18 of the cycle. All treatments were administered by an intraluteal microdialysis system (MDS). Drugs were infused for 4-hr periods on the designated days, and the treatment periods were preceded and followed by 4-hr control periods. Perfusate and jugular blood samples were collected at half-hour intervals. Perfusate samples were analyzed for progesterone (P4), oxytocin (OT), prostaglandin F2alpha (PGF2alpha), and leukotriene C4 (LTC4); jugular plasma samples were analyzed for P4, OT, and LH. Perfusion of L-NAME on Day 11 or 12 consistently increased P4 concentration in the perfusate, but had no effect on the life span of the CL. Perfusion of L-NAME on Days 17-18 also elevated P4 levels in the perfusate, and in addition, maintained P4 levels in the plasma of three of the four treated animals through Day 25 of the cycle. L-NAME perfusion also increased OT release concomitant with P4 into the perfusate at both the mid- and late-luteal phase treatments. For the most part, concentrations of LH, OT, and P4 in the jugular plasma samples collected during the perfusions were unaffected by treatments. L-NAME perfusion caused small, but significant (P < 0.05) increases in perfusate PGF2alpha and LTC4 at Days 17 and 18 and in LTC4 on Day 11 or 12. These data indicate that NO plays a direct luteolytic role in regression of the bovine CL.
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