石菖蒲叶及愈伤组织提取物的保肝活性。

N. Reshi, Sudarshana Mysore Shankarsingh, Girish Vasanaika Hodiyala
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引用次数: 1

摘要

目的:以HepG2细胞株为实验对象,研究石菖蒲叶及其愈伤组织提取物对酒精毒性的体外保护作用。材料与方法:分别在添加不同生长素的Murashige和Skoog固体培养基上培养叶片。在确定其肝保护作用之前,对其叶片和愈伤组织提取物进行了毒性剂量研究。MTT法测定细胞存活率,测定提取物的保肝作用。对叶片和愈伤组织提取物进行了初步的植物化学分析。结果:在添加2mg /L 2,4- d的MS培养基中愈伤组织形成率最高(94%)。用不同浓度(低于中毒剂量)的叶片和愈伤组织提取物预处理HepG2细胞72小时。其次是酒精中毒。结果表明,水飞蓟素预处理的HepG2细胞存活率为81%,水飞蓟素预处理的HepG2细胞存活率为90%。叶片愈伤组织提取物也显示出显著的肝保护作用,乙醇愈伤组织提取物预处理HepG2细胞后,酒精中毒后的存活率为82%。结果显示HepG2细胞存活率呈剂量依赖性。植物化学研究表明,叶片和愈伤组织提取物中存在不同的次生代谢物。结论:生物功效研究证实了其具有保肝作用的次生代谢产物。愈伤组织介导的组织显示出肝脏保护作用,这为大量生产所需的生物活性原理铺平了道路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Hepatoprotective activity of leaf and leaf callus extracts of orthosiphon aristatus (Blume) Miq.
Aim: The study was carried out to evaluate the in vitro hepatoprotective activity of leaf and leaf callus extracts of Orthosiphon aristatus against alcohol induced toxicity using HepG2 cell line. Materials and Methods: Leaf segments were cultured on Murashige and Skoog solid medium fortified with different auxins alone and in combination. Prior to the determination of hepatoprotective property leaf and leaf callus extracts were subjected to the toxic dose study. The degree of hepatoprotection of extracts was determined by measuring cell viability percentage by MTT assay. Leaf and leaf callus extracts were subjected to the preliminary phytochemical analysis. Results: Maximum percentage of callus formation (94%) was obtained in MS medium augmented with 2 mg/L of 2,4-D. HepG2 cells were pretreated with the different concentrations (below toxic dose) of leaf and leaf callus extracts for 72 hrs. followed by alcohol intoxication. Results revealed that aqueous leaf extract pretreated HepG2 cells show 90% cell viability compared to the standard silymarin pretreated HepG2 cells which showed 81% cell viability. Leaf callus extracts also showed significant hepatoprotective activity where ethanolic callus extract pretreated HepG2 cells showed82% viability after intoxication with alcohol. Results revealed that HepG2 cell viability percentage is dose dependent. Phytochemical studies revealed the presence of different secondary metabolites in leaf and leaf callus extracts. Conclusion: The bio-efficacy study confirms the presence of secondary metabolites of hepatoprotective nature. Callus mediated tissues show hepatoprotection which paves a way for the mass production of desired biologically active principles.
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