基于体鳞、颜色和贮存条件的爪哇吐舌眼镜蛇毒液的电泳谱和蛇毒磷脂酶A2 (svPLA2)活性差异

N. Kurniawan, Dea Jolie Chrestella, Dinda Sherlyndra Hapsari, Fatchiyah
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引用次数: 0

摘要

爪哇吐舌眼镜蛇(爪哇吐舌眼镜蛇)是印度尼西亚一种具有重要医学意义的蛇,背部有各种各样的鳞片颜色。本研究旨在研究不同背鳞颜色的sputamatrix毒液一般形态及其磷脂酶a2 (svPLA 2)活性的差异,并研究sputamatrix svPLA 2在不同储存条件下的活性。在东爪哇共采集了6只背鳞片颜色各异的褐背蜂。毒液在-80、4和37℃条件下保存,最长保存时间为14天。分别采用15% SDS-PAGE和蛋黄底物酸化法检测毒液谱和磷脂酶a2活性。采用统计学方法评价不同背色和不同储存条件下svPLA 2的活性。只有在某些背色蛇的毒液中才发现16 - 22 kDa的蛋白质条带。37℃时的蛋白条带强度是其他组中最轻的。褐背毛蚶的svPLA 2活性最高。贮藏温度因子和贮存周期因子的交互作用对贮藏后svpla2活性有影响。与对照组和其他试验组相比,37℃贮藏对svPLA 2活性的影响有所下降。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Differences of Electrophoretic Profile and Snake Venom Phospholipase A2 (svPLA2) Activity from the Venom of Javan Spitting Cobra, Naja sputatrix, based on Body Scales Color and Storage Condition
: Naja sputatrix (Javan spitting cobra) is one of medically important snake species in Indonesia which have various dorsal scales color. This research purposes to examine the differences of venom general profile and its phospholipase A 2 (svPLA 2 ) activity from some N. sputatrix with different dorsal scales colors, and to examine the activity of N. sputatrix svPLA 2 in different storage conditions. A total of 6 N. sputatrix from East Java with various dorsal scales color were milked. Venom storage was performed at -80, 4 and 37°C in a maximum period of 14 days. Venom profile and phospholipase A 2 activity were examined through 15% SDS-PAGE and acidimetric method using egg yolk substrate respectively. Statistical analyses were performed to evaluate svPLA 2 activity in every dorsal color and storage condition. Few protein bands range in 16 – 22 kDa are only found in the venom of the certain dorsal color snake. Protein bands at 37°C were found to have the lightest intensity among other groups. The svPLA 2 activity of brown dorsal N. sputatrix is found as the highest activity. An interaction between the storage temperature factor and period factor has effects on post-storage svPLA 2 activity. Storage in 37°C effects on svPLA 2 activity declining compared to the control group and other experimental groups.
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