基于ewood的数字微流体半月板辅助磁珠捕获用于特定蛋白质定位

TRANSDUCERS 2007 - 2007 International Solid-State Sensors, Actuators and Microsystems Conference Pub Date : 2007-06-10 DOI:10.1109/SENSOR.2007.4300228

G. J. Shah, E. Pierstorff, D. Ho, C. Kim

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引用次数: 5

摘要

本文提出了一种利用磁珠(mb)在电介质电润湿(EWOD)操作的数字(液滴)微流体中定位特定蛋白质的技术。我们确定了数字微流控和连续微流控在利用磁珠方面的根本区别，并在EWOD装置上演示了该技术。我们还展示了抗体偶联的mb(“MB-Abs”)上特定的、局部的目标蛋白浓度作为概念的证明。

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Meniscus-Assisted Magnetic Bead Trapping on Ewod-Based Digital Microfluidics for Specific Protein Localization

This paper presents a technique to localize specific proteins utilizing magnetic beads (MBs) in digital (droplet) microfluidics, operated by electrowetting-on-dielectric (EWOD). We identify the fundamental differences between digital and continuous microfluidics in utilizing magnetic beads, and demonstrate the technique on an EWOD device. We also show specific, localized concentration of a target protein over antibody- conjugated MBs ("MB-Abs") as proof of concept.

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TRANSDUCERS 2007 - 2007 International Solid-State Sensors, Actuators and Microsystems Conference

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