用玻璃微结构波导直接检测鼠疫耶尔森氏菌抗体评价鼠疫疫苗接种个体血清转化的快速方法

O. M. Kudryavtseva, Y. Skibina, V. A. Kozhevnikov, S. Bugorkova, M. V. Chainikov, А. Y. Gryaznov, D. E. Glukhov, S. S. Konnova, A. A. Shuvalov, T. N. Shchukovskaya
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引用次数: 0

摘要

的相关性。作为确保卫生和流行病学健康的一部分,在俄罗斯联邦自然鼠疫疫源地境内,根据流行病学迹象,为人口接种了预防这种感染的疫苗。由于缺乏评估疫苗接种有效性的统一方案,因此需要开发通用的表达方法,以便进行抗鼠疫免疫的筛选研究,包括在实地进行的研究。目的评价利用空心微结构玻璃波导(MGW HC)作为免疫传感器直接检测生物材料中鼠疫耶尔森氏菌抗体的表达法在鼠疫接种人血清转化动力学研究中的有效性。材料和方法。本研究采集了30名按流行指征接种鼠疫活疫苗的个体和30名未接种鼠疫疫苗且无鼠疫媒介接触史的志愿者的血清样本。在监测里海沙洲自然鼠疫疫源地居民中接种疫苗个体的框架内,根据收到的血清,评估了在不同时间间隔使用MGW HC直接检测抗体方法的可能性。在ELISA和MGW HC的帮助下,研究人员发现接种疫苗的志愿者血清中存在特异性的F1蛋白抗体。指出了直接检测抗体的速度(每次检测最多2分钟),不需要使用物种特异性二抗体,酶和底物,以及费力的样品制备的额外成本。利用玻璃微结构波导直接检测抗体的方法有望被引入到评估鼠疫疫苗免疫效果的表达方法列表中。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Direct Detection of Antibodies to Yersinia pestis Using Glass Microstructural Waveguides as an Express Method for Assessing Seroconversion in Individuals Vaccinated against Plague
Relevance. As part of ensuring sanitary and epidemiological well­-being, in the territory of natural plague foci of the Russian Federation, according to epidemiological indications, the population is vaccinated against this infection. The lack of a unified scheme for evaluating the effectiveness of vaccination dictates the need to develop universal express methods that allow screening studies of anti­-plague immunity, including in the field.Aims. To evaluate the effectiveness of the express method for the direct detection of antibodies to Yersinia pestis in biological material using microstructural glass waveguides with a hollow core (MGW HC) as immunosensors in studying the dynamics of seroconversion in people vaccinated with the plague live people.Materials and methods. In the study, blood serum samples were taken from 30 individuals vaccinated according to epidemic indications with the live plague vaccine (PLV) and 30 volunteers who were not vaccinated and did not have a history of contact with the plague agent.Results. An assessment was made of the possibility of using the method of direct detection of antibodies using MGW HC at different time intervals according to the receipt of blood sera in the framework of monitoring vaccinated individuals from among the inhabitants of the Caspian sandy natural plague focus. The presence of specific antibodies to the F1 protein in the blood serum of vaccinated volunteers was revealed both with the help of ELISA and with the use of MGW HC. The speed of the method of direct detection of antibodies (maximum 2 minutes per test), the absence of the need to use species­specific secondary antibodies, enzymes and substrates and additional costs for laborious sample preparation were noted.Conclusions. The method of direct detection of antibodies using glass microstructural waveguides is promising for introduction into the list of express methods for assessing the immunological effectiveness of anti­plague vaccination.
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