基于薄膜衍生金纳米粒子(Au NPs)表面等离子体共振(SPR)检测前列腺特异性抗原(PSA)的初步研究

Sensor Letters Pub Date : 2020-11-01 DOI:10.1166/sl.2020.4286

M. Dhanunjaya, T. Sonklin, Pimchanok Leuasoongnoen, S. Suksaweang, P. Janphuang, S. Pojprapai

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引用次数: 0

摘要

研究了基于金纳米粒子(Au NPs)局部表面等离子体共振(LSPR)的前列腺特异性抗原(PSA)生物标志物检测方法。采用直流磁控溅射法制备了Si和石英衬底上的金纳米粒子。用简单的下降成本法评价了Au NPs对抗体和PSA的功能化作用。SPR峰强度与PSA浓度成正比。SPR峰位置的红移可能是由于Au NPs周围的折射率随着抗体的偶联和不同浓度特异性PSA的进一步结合而发生变化。

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Initial Study of Thin-Film Derived Gold Nanoparticles (Au NPs) Surface Plasmon Resonance (SPR) Based Prostate-Specific Antigen (PSA) Detection

The gold nanoparticles (Au NPs) localized surface plasmon resonance (LSPR) based detection of prostate-specific antigen (PSA) biomarker was carried out in this work. Au NPs on Si and quartz substrates were synthesized by DC magnetron sputtering method followed by conventional annealing protocol. The functionalization of antibody and PSA with Au NPs was evaluated using a simple drop-cost method. The SPR peak intensity was found to be proportional to the PSA concentration. The redshift in SPR peak position might be owing to the change in refractive index around the Au NPs with the conjugation of antibody and further additional binding of specific PSA at different concentrations.

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来源期刊

Sensor Letters 工程技术-电化学

自引率

0.00%

发文量

审稿时长

6 months

期刊介绍： The growing interest and activity in the field of sensor technologies requires a forum for rapid dissemination of important results: Sensor Letters is that forum. Sensor Letters offers scientists, engineers and medical experts timely, peer-reviewed research on sensor science and technology of the highest quality. Sensor Letters publish original rapid communications, full papers and timely state-of-the-art reviews encompassing the fundamental and applied research on sensor science and technology in all fields of science, engineering, and medicine. Highest priority will be given to short communications reporting important new scientific and technological findings.