基于紫外导数光谱法同时分析三水合头孢克肟和盐酸氨溴索的绿色分析方法

Ceema Mathew, Suman Naik Bhukya, A. Makula, R. Puvvadi
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引用次数: 0

摘要

背景:到目前为止,还没有关于三水合头孢克肟(CEF)和盐酸氨溴索(ABH)联合使用的导数分光光度法的报道。因此,迫切需要开发一种紫外导数光谱方法,以减少与高效液相色谱法或高效薄层色谱法相比的分析成本。目的:建立和验证一种经济、环保的导数光谱方法,避免使用有机溶剂同时定量两种药物。材料与方法:建立了一种基于零交叉波长导数分光光度法的同时定量CEF和ABH的简便方法。由于该方法依赖于亲水溶解,因此采用0.1N尿素作为溶剂,是一种经济环保的方法。分别选择波长253 nm (CEF为零交叉点)和306 nm (ABH为零交叉点)对ABH和CEF进行定量。结果:CEF和ABH在5 ~ 35 μg/ml和3 ~ 10.5 μg/ml范围内呈线性关系,检测限分别为0.187和0.0937 μg/ml,定量限分别为0.625和0.312 μg/ml。回收率在99.05% ~ 102%之间。该方法精密度和准确度的相对标准偏差<2%。结论:该方法简便、准确、精密度高,可成功应用于所研究药物片剂的常规质量控制分析。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A green analytical method for the simultaneous analysis of cefixime trihydrate and ambroxol HCl based on ultraviolet derivative spectroscopy
Context: Until date, there is no reported derivative spectrophotometric method for the combination of cefixime trihydrate (CEF) and ambroxol HCl (ABH). So an urgent need was felt to develop an ultraviolet (UV) derivative spectroscopic method, which reduces the cost of analysis on comparing with high-performance liquid chromatography or high-performance thin layer chromatography method. Aims: To develop and validate an economical and ecofriendly derivative spectroscopic method that avoids the use of organic solvents for simultaneous quantification of both the drugs. Materials and Methods: A simple method based on the derivative spectrophotometric method at zero crossing wavelengths has been developed for the simultaneous quantification of CEF and ABH. As the method depends on hydrotropic dissolution, 0.1N urea is used as the solvent, and it yields an economical and ecofriendly method. Two wavelengths 253 nm (zero crossing point (ZCP) for CEF) and 306 nm (ZCP for ABH) were selected for the quantification of ABH and CEF respectively. Results: The first derivative amplitude-concentration plots were linear over the range of 5-35 μg/ml and 3-10.5 μg/ml with detection limits of 0.187 and 0.0937 μg/ml and quantification limits of 0.625 and 0.312 μg/ml for CEF and ABH respectively. The percentage recovery was within the range between 99.05% and 102%. The % relative standard deviation for precision and accuracy of the method was found to be <2%. Conclusion: The proposed method was found to be simple, accurate and precise and can be successfully applied to the routine quality control analysis of studied drugs in their tablet formulations.
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