Development of Methods For Quantitative Determination of Polyphenols in Grass Pentaphylloides Fruticosa (L.) O. Schwarz.

The purpose of this study was to develop a method of qualitative and quantitative assessment of biologically active substances of P. fruticosa L. herb. As object of study was used P. fruticosa L. herb. We used the method of high performance liquid chromatography in reversed-phase variant. As a result of chromatography, it was found that the chromatographic system used can effectively separate and identify the polyphenol complex of the herb P. fruticosus L. The chromatogram shows that the polyphenol complex of the herb P. fruticosus L. consists of the glycosides quercetin, kaempferol, rhamnetin, procyanidins, ellagotannins and ellagic acid and chlorogenic acid. Aglycones were found in the hydrolysis products: quercetin, kempferol, ellagic acid, and trace amounts of rhamnetin. Thus, as a result of this study, the composition of the polyphenolic complex of the herb P. fruticosus L. was established, including the glycosides of quercetin, kaempferol, rhamnetin, proacyanidins, ellagic acid and ellagotannins. A method has been developed for the quantitative determination of the sum of aglycones of grass polyphenols of P. fruticosus L., which is based on the principle of extraction with the simultaneous hydrolysis of glycosides of polyphenols to aglycones and their subsequent chromatographic determination by high performance liquid chromatography and quantification using absolute calibration. Keywords—P. fruticosa L. herb, RP HPLC, quercetin glycosides, kaempferol glycosides, rhamnetin glycosides, proacyanidins, elagic acid, ellagotannins