禾草五味子中多酚含量测定方法的建立o·施瓦兹。

E. Zhilyakova, O. Novikov, D. Pisarev, L. Zolotareva
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引用次数: 0

摘要

本研究的目的是建立一种定性和定量评价水仙草生物活性物质的方法。以金银花为研究对象。采用高效液相色谱法进行反相变异。通过色谱分析,发现所采用的色谱体系能够有效地分离和鉴定枸杞多酚复合物。色谱图显示,枸杞多酚复合物由槲皮素、山奈酚、鼠李素、原花青素、鞣花单宁、鞣花酸和绿原酸等苷类组成。水解产物中含有槲皮素、肯普酚、鞣花酸和微量鼠李素等苷元。因此,通过本研究,确定了该植物多酚复合物的组成,包括槲皮素、山奈酚、鼠李素、原花青素、鞣花酸和鞣花单宁。采用高效液相色谱法提取多酚中的苷类,同时水解成苷元,再用绝对定标法进行色谱测定,建立了草多酚中苷元数量的定量测定方法。Keywords-P。反相高效液相色谱法,槲皮素苷,山奈酚苷,鼠李素苷,原花青素,鞣花酸,鞣花单宁
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Development of Methods For Quantitative Determination of Polyphenols in Grass Pentaphylloides Fruticosa (L.) O. Schwarz.
The purpose of this study was to develop a method of qualitative and quantitative assessment of biologically active substances of P. fruticosa L. herb. As object of study was used P. fruticosa L. herb. We used the method of high performance liquid chromatography in reversed-phase variant. As a result of chromatography, it was found that the chromatographic system used can effectively separate and identify the polyphenol complex of the herb P. fruticosus L. The chromatogram shows that the polyphenol complex of the herb P. fruticosus L. consists of the glycosides quercetin, kaempferol, rhamnetin, procyanidins, ellagotannins and ellagic acid and chlorogenic acid. Aglycones were found in the hydrolysis products: quercetin, kempferol, ellagic acid, and trace amounts of rhamnetin. Thus, as a result of this study, the composition of the polyphenolic complex of the herb P. fruticosus L. was established, including the glycosides of quercetin, kaempferol, rhamnetin, proacyanidins, ellagic acid and ellagotannins. A method has been developed for the quantitative determination of the sum of aglycones of grass polyphenols of P. fruticosus L., which is based on the principle of extraction with the simultaneous hydrolysis of glycosides of polyphenols to aglycones and their subsequent chromatographic determination by high performance liquid chromatography and quantification using absolute calibration. Keywords—P. fruticosa L. herb, RP HPLC, quercetin glycosides, kaempferol glycosides, rhamnetin glycosides, proacyanidins, elagic acid, ellagotannins
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