骨不连实验研究动物模型的建立

Journal of Sun Yat-sen University Pub Date : 2002-09-20 DOI:10.13471/J

Lin Bo-wen, Xu Zhong-shi, X. De-ming, LU X iao-fu, Yang Da-zhi

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引用次数: 9

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Establishment of an Animal Model for Experimental Study of Bone Nonunion

目的：对人类组织相容性抗原（ＨｕｍａｎＬｅｕｃｏｃｙｔｅＡｎｔｉｇｅｎＳｙｓｔｅｍ）Ⅱ类进行高分辨基因分析。方法：用多重ＰＣＲ和等位基因特异性ＰＣＲ技术相结合＾［１，２］，建立多重等位基因特异性。结果：２４对引物成功在分辨４３份ＤＮＡ的ＨＬＡ－ＤＲＢ１、Ｂ３、Ｂ４、Ｂ５＾＊５１个等位基因和ＱＤＢ１＾＊１５个等位基因的４位数字特异性，识别基因片段长度为２８１ｂｐｏ。结论：多重等位基因特异性ＰＣ

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Journal of Sun Yat-sen University

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