人肿瘤抑制蛋白101F6重组成磷脂双层纳米圆盘的分子功能研究

KIMIKA Pub Date : 2019-06-03 DOI:10.26534/kimika.v30i1.1-3

Mohammed El Behery, A. Asada, F. Takeuchi, Tetsunari Kimura, E. Chatani, M. Tsubaki

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摘要

人肿瘤抑制基因101F6候选产物在毕赤酵母细胞中成功表达。采用两种重组方法将纯化的101F6蛋白成功地整合到不同大小的磷脂双层纳米圆盘中;自组装和重组成预制的空纳米片。重组的101F6蛋白可以被抗坏血酸快速还原，即使在室温下也非常稳定。

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Elucidation of molecular functions of human tumor suppressor protein 101F6 by reconstitution into phospholipid bilayer nanodiscs

A candidate human tumor suppressor gene 101F6 product was expressed successfully in Pichia pastoris yeast cells. The purified 101F6 protein was successfully incorporated into phospholipid bilayer nanodiscs with different sizes by employing two reconstitution methods; self-assembly and reconstitution into the preformed empty nanodisc. The reconstituted 101F6 protein could be reduced with ascorbate quickly and was very stable even at ambient temperatures.

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