人致病性金黄色葡萄球菌多药耐药率及其对泻泻草叶提取物的敏感性

MD AL Nayem Chowdhury, Nazmul Hossain, Mahbubur Rahman, Ashrafuzzaman
{"title":"人致病性金黄色葡萄球菌多药耐药率及其对泻泻草叶提取物的敏感性","authors":"MD AL Nayem Chowdhury, Nazmul Hossain, Mahbubur Rahman, Ashrafuzzaman","doi":"10.3329/ICPJ.V2I11.16525","DOIUrl":null,"url":null,"abstract":"Staphylococcus aureus is one of the major pathogen responsible for skin infection, urinary tract infection (UTI) and endocarditis in human. The study was performed to determine the prevalence of multidrug resistant S. aureus in human clinical sample and to evaluate their sensitivity to Allamanda cathartica L. leaf extract. A total of 12 isolates were identified belongs to S. aureus by performing several physiological and biochemical tests. The isolates exhibited highest resistant (75%) to streptomycin and lowest (33.33%) against co-trimoxazole followed by disc diffusion assay of eight antibiotics tested. The other four antibiotics such as azithromycin, chloramphenicol, gentamycin and erythromycin exhibited 50 to 66.67% resistant to present isolates. Here we found that 75% of S. aureus isolates were multidrug-resistant (MDR). The crude leaf extract of A. cathartica L. found to possess antibacterial properties at the rate of 83.33% against S. aureus isolates with 12-22 mm zone of inhibition. Results of TLC states that Benzene : Ethyl acetate (1:1) solvent system was more effective for initial separation of compound from crude leaf extract resulted three distinct bands with different Rf values ranging from 0.53 to 0.89. The result of this study refers that A. cathartica L. leaf extract would be useful to develop effective drugs that would reduce the higher prevalence of multidrug resistance S. aureus causing clinical infection in human.","PeriodicalId":13811,"journal":{"name":"International Current Pharmaceutical Journal","volume":"39 1","pages":"185-188"},"PeriodicalIF":0.0000,"publicationDate":"2013-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Prevalence of multidrug resistance in human pathogenic Staphylococcus aureus and their sensitivity to Allamanda cathartica L. leaf extract\",\"authors\":\"MD AL Nayem Chowdhury, Nazmul Hossain, Mahbubur Rahman, Ashrafuzzaman\",\"doi\":\"10.3329/ICPJ.V2I11.16525\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Staphylococcus aureus is one of the major pathogen responsible for skin infection, urinary tract infection (UTI) and endocarditis in human. The study was performed to determine the prevalence of multidrug resistant S. aureus in human clinical sample and to evaluate their sensitivity to Allamanda cathartica L. leaf extract. A total of 12 isolates were identified belongs to S. aureus by performing several physiological and biochemical tests. The isolates exhibited highest resistant (75%) to streptomycin and lowest (33.33%) against co-trimoxazole followed by disc diffusion assay of eight antibiotics tested. The other four antibiotics such as azithromycin, chloramphenicol, gentamycin and erythromycin exhibited 50 to 66.67% resistant to present isolates. Here we found that 75% of S. aureus isolates were multidrug-resistant (MDR). The crude leaf extract of A. cathartica L. found to possess antibacterial properties at the rate of 83.33% against S. aureus isolates with 12-22 mm zone of inhibition. Results of TLC states that Benzene : Ethyl acetate (1:1) solvent system was more effective for initial separation of compound from crude leaf extract resulted three distinct bands with different Rf values ranging from 0.53 to 0.89. The result of this study refers that A. cathartica L. leaf extract would be useful to develop effective drugs that would reduce the higher prevalence of multidrug resistance S. aureus causing clinical infection in human.\",\"PeriodicalId\":13811,\"journal\":{\"name\":\"International Current Pharmaceutical Journal\",\"volume\":\"39 1\",\"pages\":\"185-188\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2013-10-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Current Pharmaceutical Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3329/ICPJ.V2I11.16525\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Current Pharmaceutical Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3329/ICPJ.V2I11.16525","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

金黄色葡萄球菌(Staphylococcus aureus)是人类皮肤感染、尿路感染和心内膜炎的主要病原体之一。本研究旨在确定人类临床样品中耐多药金黄色葡萄球菌的流行情况,并评估其对泻泻草叶提取物的敏感性。经多项生理生化试验鉴定,共分离出12株金黄色葡萄球菌。对链霉素耐药率最高(75%),对复方新诺明耐药率最低(33.33%)。其余4种抗生素如阿奇霉素、氯霉素、庆大霉素和红霉素对该菌株的耐药率为50% ~ 66.67%。我们发现75%的金黄色葡萄球菌分离株具有多重耐药(MDR)。对金黄色葡萄球菌的抑菌率为83.33%,抑菌带为12 ~ 22 mm。薄层色谱结果表明,苯:乙酸乙酯(1:1)溶剂体系对粗叶提取物的初始分离效果较好,有三条不同的条带,Rf值在0.53 ~ 0.89之间。本研究结果为开发有效的药物,降低耐多药金黄色葡萄球菌在人类临床感染中的较高流行率提供了参考。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Prevalence of multidrug resistance in human pathogenic Staphylococcus aureus and their sensitivity to Allamanda cathartica L. leaf extract
Staphylococcus aureus is one of the major pathogen responsible for skin infection, urinary tract infection (UTI) and endocarditis in human. The study was performed to determine the prevalence of multidrug resistant S. aureus in human clinical sample and to evaluate their sensitivity to Allamanda cathartica L. leaf extract. A total of 12 isolates were identified belongs to S. aureus by performing several physiological and biochemical tests. The isolates exhibited highest resistant (75%) to streptomycin and lowest (33.33%) against co-trimoxazole followed by disc diffusion assay of eight antibiotics tested. The other four antibiotics such as azithromycin, chloramphenicol, gentamycin and erythromycin exhibited 50 to 66.67% resistant to present isolates. Here we found that 75% of S. aureus isolates were multidrug-resistant (MDR). The crude leaf extract of A. cathartica L. found to possess antibacterial properties at the rate of 83.33% against S. aureus isolates with 12-22 mm zone of inhibition. Results of TLC states that Benzene : Ethyl acetate (1:1) solvent system was more effective for initial separation of compound from crude leaf extract resulted three distinct bands with different Rf values ranging from 0.53 to 0.89. The result of this study refers that A. cathartica L. leaf extract would be useful to develop effective drugs that would reduce the higher prevalence of multidrug resistance S. aureus causing clinical infection in human.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信