{"title":"人体红细胞对正磷酸盐离子的渗透性","authors":"Josette Chedru, Pierre Cartier","doi":"10.1016/0926-6585(66)90008-2","DOIUrl":null,"url":null,"abstract":"<div><p>The kinetics of the influx of PO<sub>4</sub><sup>3−</sup> into erythrocytes reveal a first-order process, with a half-time of 140 min at 37°.</p><p>The exchange measured for short times of incubation (0–300 sec) revealed that the incorporation of <sup>32</sup>P begins immediately and increases linearly. Inorganic phosphate is the precursor of phosphate in organic fractions: the highest initial specific activity was constantly found in this inorganic fraction. Incorporation of labelled phosphate into ATP and ADP is not so rapid and phosphate esters exchange very slowly.</p><p>The flux through the membrane remains proportional to the extracellular concentration and is not affected by a ten-fold increase above physiological conditions. This lack of saturation effect and null value for the affinity constant, excludes any participation of an active site located in the membrane.</p><p>The kinetics of permeability are not modified by competitive anions (SO<sub>4</sub><sup>2−</sup>, AsO<sub>4</sub><sup>3−</sup>). The AsO<sub>4</sub><sup>3−</sup> ions decreased the uptake slowly; however, this effect is an indirect consequence of the inhibition of glycolysis.</p><p>The above results are in accordance with a process of simple diffusion.</p></div>","PeriodicalId":100158,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis","volume":"126 3","pages":"Pages 500-512"},"PeriodicalIF":0.0000,"publicationDate":"1966-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6585(66)90008-2","citationCount":"10","resultStr":"{\"title\":\"La perméabilité des globules rouges humains aux ions orthophosphates\",\"authors\":\"Josette Chedru, Pierre Cartier\",\"doi\":\"10.1016/0926-6585(66)90008-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The kinetics of the influx of PO<sub>4</sub><sup>3−</sup> into erythrocytes reveal a first-order process, with a half-time of 140 min at 37°.</p><p>The exchange measured for short times of incubation (0–300 sec) revealed that the incorporation of <sup>32</sup>P begins immediately and increases linearly. Inorganic phosphate is the precursor of phosphate in organic fractions: the highest initial specific activity was constantly found in this inorganic fraction. Incorporation of labelled phosphate into ATP and ADP is not so rapid and phosphate esters exchange very slowly.</p><p>The flux through the membrane remains proportional to the extracellular concentration and is not affected by a ten-fold increase above physiological conditions. This lack of saturation effect and null value for the affinity constant, excludes any participation of an active site located in the membrane.</p><p>The kinetics of permeability are not modified by competitive anions (SO<sub>4</sub><sup>2−</sup>, AsO<sub>4</sub><sup>3−</sup>). The AsO<sub>4</sub><sup>3−</sup> ions decreased the uptake slowly; however, this effect is an indirect consequence of the inhibition of glycolysis.</p><p>The above results are in accordance with a process of simple diffusion.</p></div>\",\"PeriodicalId\":100158,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis\",\"volume\":\"126 3\",\"pages\":\"Pages 500-512\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1966-11-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0926-6585(66)90008-2\",\"citationCount\":\"10\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0926658566900082\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926658566900082","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
La perméabilité des globules rouges humains aux ions orthophosphates
The kinetics of the influx of PO43− into erythrocytes reveal a first-order process, with a half-time of 140 min at 37°.
The exchange measured for short times of incubation (0–300 sec) revealed that the incorporation of 32P begins immediately and increases linearly. Inorganic phosphate is the precursor of phosphate in organic fractions: the highest initial specific activity was constantly found in this inorganic fraction. Incorporation of labelled phosphate into ATP and ADP is not so rapid and phosphate esters exchange very slowly.
The flux through the membrane remains proportional to the extracellular concentration and is not affected by a ten-fold increase above physiological conditions. This lack of saturation effect and null value for the affinity constant, excludes any participation of an active site located in the membrane.
The kinetics of permeability are not modified by competitive anions (SO42−, AsO43−). The AsO43− ions decreased the uptake slowly; however, this effect is an indirect consequence of the inhibition of glycolysis.
The above results are in accordance with a process of simple diffusion.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
