增强外周再生作为潜在的糖尿病神经病变治疗靶点的风疹蚓提取物DLBS1033N:细胞活力和迁移的作用

IF 0.7 Q4 PHARMACOLOGY & PHARMACY
Yesiska Kristina Hartanti, A. Nugroho, R. Tjandrawinata
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引用次数: 0

摘要

糖尿病周围神经病变(DPN)显著影响生活质量,目前尚无明确的治疗方法。鉴于DPN治疗的病理基础,促进神经元再生的同时限制神经退行性变至关重要。对外周再生起关键作用的雪旺细胞在糖尿病患者中表现为细胞增殖和生存抑制,外周再生能力持续下降。DLBS1033N是一种从风疹蚓中提取的蛋白水解物,经证实可通过磷脂酰肌醇-3激酶(PI3K)途径诱导神经生长因子(NGF)的表达,从而促进许旺细胞系RSC96的生长和存活。该通路对雪旺细胞的增殖和迁移有重要作用。本文研究了DLBS1033N对高糖(50mM)诱导的大鼠雪旺细胞系RSC96损伤的外周再生的贡献,这是一种著名的DPN体外细胞模型。分别用高糖(50mM)处理RSC96,添加或不添加25、50、100μg/mL的DLBS1033N处理24、48、72 h,采用MTS检测试剂盒评估细胞活力。DLBS1033N在48 h的培养时间内显著促进细胞增殖,且呈剂量依赖性(p < 0.05)。此外,100μg/ml的DLBS1033N在培养48 H后显著促进细胞迁移16% (p < 0.05),有利于细胞外周再生。综上所述,DLBS1033N可促进外周再生,可作为一种有效且有前景的DPN治疗方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Enhancement Peripheral Regeneration as a Target of Potential Diabetic Neuropathy Treatment from Lumbricus rubellus Fraction DLBS1033N: the role of cell viability and migration
Diabetic Peripheral Neuropathy (DPN) significantly affects the quality of life with no definitive therapy currently. Given the pathologic basis for DPN treatment, it's critical to promote neuron regeneration while also restricting nerve degeneration. Schwann cells that play pivotal roles against peripheral regeneration manifest cell proliferation and survival inhibition in diabetic patients consecutively decreased peripheral regeneration capacity. DLBS1033N, a protein hydrolysate obtained from Lumbricus rubellus, has been confirmed to promote Schwann cell line RSC96 growth and survival by induction Nerve Growth Factor (NGF) expression via phosphatidylinositol-3‑kinase (PI3K) pathway. This pathway has an important contribution against Schwann cell proliferation and migration. Herein, the contribution of DLBS1033N to peripheral regeneration on high-glucose (50mM)-induced rat Schwann cell line RSC96 injury, a well-known DPN in vitro cell model. RSC96 were treated with high glucose (50mM) with or without DLBS1033N 25, 50, and 100μg/mL for 24, 48, and 72 h. MTS assay kit were used to evaluate cell viability. DLBS1033N significantly improved cell proliferation in 48 h incubation time with a dose-dependent manner (p < 0.05). Furthermore, DLBS1033N 100μg/ml significantly promoted cell migration by 16% in 48 H incubation (p < 0.05) determined by scratch assay, as the beneficial action to accomplish peripheral regeneration. In conclusion, DLBS1033N enhanced peripheral regeneration which could be used as an effective and promising DPN treatment.
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来源期刊
INDONESIAN JOURNAL OF PHARMACY
INDONESIAN JOURNAL OF PHARMACY PHARMACOLOGY & PHARMACY-
CiteScore
1.20
自引率
0.00%
发文量
38
审稿时长
12 weeks
期刊介绍: The journal had been established in 1972, and online publication was begun in 2008. Since 2012, the journal has been published in English by Faculty of Pharmacy Universitas Gadjah Mada (UGM) Yogyakarta Indonesia in collaboration with IAI (Ikatan Apoteker Indonesia or Indonesian Pharmacist Association) and only receives manuscripts in English. Indonesian Journal of Pharmacy is Accredited by Directorate General of Higher Education. The journal includes various fields of pharmaceuticals sciences such as: -Pharmacology and Toxicology -Pharmacokinetics -Community and Clinical Pharmacy -Pharmaceutical Chemistry -Pharmaceutical Biology -Pharmaceutics -Pharmaceutical Technology -Biopharmaceutics -Pharmaceutical Microbiology and Biotechnology -Alternative medicines.
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