利用ISSR-PCR标记分析乌克兰水牛种群的遗传结构

N. Mokhnachova
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引用次数: 1

摘要

由于农业动物生物多样性的减少,对牛(包括牛亚科Bubalus属)种内遗传多样性的研究具有重要意义。遗传多样性的基础是其遗传成分。本地物种和牛种的丧失是对生物圈的真正威胁,因为自然生态系统和农业生态系统的复原力与其适应环境条件的遗传能力直接相关。对来自“TASBIO”LLC (Chernihiv地区)农场的乌克兰水牛种群(Bubalus bubalis)的66只动物进行issr -标记多态性分析。用一套标准试剂从静脉血中分离基因组DNA样本。采用特异性ISSR引物进行基因分型:(ACC)6G, (GAG)6C, (AG)9C, (CTC)6C, (AG)8CA, (AG)8CGand (GA)6CC。我们通过扩增子的数量,它们的长度(核苷酸的数量)和它们的多态性来确定光谱的差异。结果表明,所有引物均表现出水牛DNA区域的多态性。扩增子的范围从200 bp到4000 bp。ISSR分析共发现87个位点,其中71个为多态性位点。(AG) 8ca标记多态性最低(PIC=0.234), (CTC)6C标记多态性最高(PIC=0.389)。在4个ISSR标记中发现保守位点:6 in (AG)8CA标记,5 in (AG)8CG标记,4 in (GA)6CC标记和1 in (AG)9C标记。在乌克兰水牛种群中鉴定出67个物种特异性位点:(AG)9C位点10个,(ACC)6G位点3个,(GAG)6C位点4个,(CTC)6C位点7个,(AG)8CA位点15个,(AG)8CG和(GA)6CC位点14个。推荐使用ISSR引物进行水牛DNA多态性的分子遗传分析。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genetic structure of the Ukrainian water buffalo population by ISSR-PCR markers
The study of intraspecific genetic diversity of cattle, including species of the genus Bubalus from the subfamily Bull, is important because of the reduction of biodiversity of farm animals. The basis of genetic diversity is its genetic component. The loss of native species and breeds of cattle is a real threat to the biosphere, as the resilience of natural ecosystems and agroecosystems is directly linked to their genetic ability to adapt to environmental conditions. Polymorphism of ISSR-markers of the Ukrainian population of water buffaloes (Bubalus bubalis) from the farm of “TASBIO” LLC (Chernihiv region) was analyzed in 66 animals selected for the study. Genomic DNA samples were isolated from venous blood with a standard set of reagents. Genotyping was performed using specific ISSR primers: (ACC)6G, (GAG)6C, (AG)9C, (CTC)6C, (AG)8CA, (AG)8CGand (GA)6CC. We determined the difference in spectra both by the number of amplicons, their lengths (number of nucleotides) and by their polymorphism. As a result of the study, all primers showed polymorphism of buffalo DNA regions. Amplicons were defined in the range from 200 bp up to 4000 bp. Analysis of ISSR spectra revealed 87 loci, of which 71 were polymorphic. (AG)8CA-marker was the least polymorphic (PIC=0.234), (CTC)6C the most polymorphic (PIC=0.389). Conservative loci were found in four ISSR markers: 6 in (AG)8CA marker, 5 in (AG)8CG, 4 in (GA)6CC, and 1 in (AG)9C. 67 species-specific loci were identified for the Ukrainian water buffalo population: 10 for (AG)9C, 3 loci for (ACC)6G, 4 for (GAG)6C, 7 for (CTC)6C, 15 for (AG)8CA, and 14 in (AG)8CG and (GA)6CC. The ISSR primers used are recommended for molecular genetic analysis of buffalo DNA polymorphism.
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