S-100蛋白在青年人和老年人小脑浦肯野细胞中的形态特征和表达

A. A. Balandin, L. M. Zheleznov, I. A. Balandina
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摘要

在现代科学文献中,对人体衰老问题和与年龄有关的疾病的预防进行了大量的研究。研究人员对小脑的衰老表现出越来越浓厚的兴趣。形成小脑皮层神经节层的浦肯野细胞被认为对各种影响非常敏感。目的是揭示人类小脑浦肯野细胞的形态功能特征,在老年与年轻的年龄进行比较。材料和方法。在解剖过程中,对59名男性和52名女性右撇子的小脑进行了形态学研究,其中的记忆数据排除了中枢和周围神经系统器官的疾病和损伤,以及酒精或药物成瘾。本研究的材料取自小脑下半月小叶区域。研究材料分为两组:第一组(n=59)包括31名男性和28名年轻女性(25至32岁)的样本,第二组(n=52)包括28名男性和24名老年女性(79至88岁)。我们采用一般和神经组织学,免疫组织化学和形态计量学的研究方法。对浦肯野细胞进行形态学评估,并测定其体间距离。免疫组织化学研究使用了一组S-100蛋白的抗体。对获得的数据进行的比较分析显示,与年轻人相比,老年人浦肯野细胞体之间的距离在统计学上具有显著优势。免疫组化研究显示,s -100免疫阳性的浦肯野细胞在老年时增多。关于人类小脑神经组织细胞结构的年龄相关结构转变获得了新的数据,在研究年龄相关疾病和运动协调障碍时必须考虑到这一点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Morphological characteristics and expression of the S-100 protein in Purkinje cells of the cerebellum of a young and senile human
The problem of aging of the human body and the prevention of age-associated diseases in modern scientific literature is devoted to many studies. Researchers are showing increased interest in the aging of the cerebellum. Purkinje cells, which form the ganglionic layer of the cerebellar cortex, are considered to be very sensitive to various influences.The aim is to reveal the morphofunctional features of the Purkinje cells of the human cerebellum in the senile age in comparison with the young age.Material and methods. Morphological study of the cerebellum of 59 men and 52 women, right-handed, with anamnestic data that excluded diseases and injuries of the organs of the central and peripheral nervous system, as well as alcohol or drug addiction, was carried out during autopsy. The material for the study was taken from the region of the inferior semilunar lobule of the cerebellum. The studied material was divided into two groups: group I (n=59) included samples obtained from 31 men and 28 young women (from 25 to 32 years old inclusive), group II (n=52) – from 28 men and 24 women of senile age (from 79 to 88 years inclusive). We used general and neurohistological, immunohistochemical and morphometric research methods. Morphological assessment of Purkinje cells was carried out and the distance between their bodies was determined. An immunohistochemical study used a panel of antibodies to the S-100 protein.Results. A comparative analysis of the data obtained showed a statistically significant predominance of the distance between the bodies of Purkinje cells in the elderly compared to the young. Immunohistochemical study revealed an increase in the number of S-100-immunopositive Purkinje cells in old age.Conclusion. New data have been obtained on the age-related structural transformation of the cytoarchitectonics of the human cerebellar nervous tissue, which must be taken into account when studying age-associated diseases and movement coordination disorders.
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