利用RAPD和SSR标记对生姜基因型进行分子鉴定

IF 0.2 Q4 HORTICULTURE

Journal of Horticultural Sciences Pub Date : 2022-09-27 DOI:10.24154/jhs.v17i1.1052

Akshitha HJ, Prasath D, Umesha K, Mohammed Faisal P, Venkataravanappa V

{"title":"利用RAPD和SSR标记对生姜基因型进行分子鉴定","authors":"Akshitha HJ, Prasath D, Umesha K, Mohammed Faisal P, Venkataravanappa V","doi":"10.24154/jhs.v17i1.1052","DOIUrl":null,"url":null,"abstract":"Genetic diversity among ginger genotypes collected from different parts of the country was studied using molecular markers (30 RAPD and 55 SSR). Compared to RAPD primers SSR primers were efficient in distinguishing the genotypes. A total of 86 and 23 polymorphic bands were observed with RAPD and SSR primers, respectively. Percentage polymorphism observed between RAPD and SSR primers was 97.40 % and 56.54 %. Grouping of genotypes by using combined data of RAPD and SSR primers indicated that irrespective of their place of collection or geographical origin, 30 genotypes were clustered into different groups which showed that, each individual genotype is having wider variability or it might be due to the genetic similarity existing among them.","PeriodicalId":36766,"journal":{"name":"Journal of Horticultural Sciences","volume":"71 1","pages":""},"PeriodicalIF":0.2000,"publicationDate":"2022-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Molecular characterization of ginger genotypes using RAPD and SSR markers\",\"authors\":\"Akshitha HJ, Prasath D, Umesha K, Mohammed Faisal P, Venkataravanappa V\",\"doi\":\"10.24154/jhs.v17i1.1052\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Genetic diversity among ginger genotypes collected from different parts of the country was studied using molecular markers (30 RAPD and 55 SSR). Compared to RAPD primers SSR primers were efficient in distinguishing the genotypes. A total of 86 and 23 polymorphic bands were observed with RAPD and SSR primers, respectively. Percentage polymorphism observed between RAPD and SSR primers was 97.40 % and 56.54 %. Grouping of genotypes by using combined data of RAPD and SSR primers indicated that irrespective of their place of collection or geographical origin, 30 genotypes were clustered into different groups which showed that, each individual genotype is having wider variability or it might be due to the genetic similarity existing among them.\",\"PeriodicalId\":36766,\"journal\":{\"name\":\"Journal of Horticultural Sciences\",\"volume\":\"71 1\",\"pages\":\"\"},\"PeriodicalIF\":0.2000,\"publicationDate\":\"2022-09-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Horticultural Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.24154/jhs.v17i1.1052\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"HORTICULTURE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Horticultural Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.24154/jhs.v17i1.1052","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"HORTICULTURE","Score":null,"Total":0}

引用次数: 0

摘要

利用分子标记(30个RAPD和55个SSR)对全国不同地区生姜基因型的遗传多样性进行了研究。与RAPD引物相比，SSR引物能有效区分基因型。RAPD和SSR引物共检测到86条和23条多态性条带。RAPD和SSR引物多态性分别为97.40%和56.54%。利用RAPD和SSR引物组合数据对基因型进行分组，结果表明，30个基因型不论其采集地点和地理来源如何，均可聚为不同的群体，说明每个基因型具有较大的变异性，也可能是由于基因型之间存在遗传相似性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Molecular characterization of ginger genotypes using RAPD and SSR markers

Genetic diversity among ginger genotypes collected from different parts of the country was studied using molecular markers (30 RAPD and 55 SSR). Compared to RAPD primers SSR primers were efficient in distinguishing the genotypes. A total of 86 and 23 polymorphic bands were observed with RAPD and SSR primers, respectively. Percentage polymorphism observed between RAPD and SSR primers was 97.40 % and 56.54 %. Grouping of genotypes by using combined data of RAPD and SSR primers indicated that irrespective of their place of collection or geographical origin, 30 genotypes were clustered into different groups which showed that, each individual genotype is having wider variability or it might be due to the genetic similarity existing among them.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Journal of Horticultural Sciences Agricultural and Biological Sciences-Plant Science

CiteScore

0.30

自引率

0.00%

发文量

审稿时长

6 weeks