大豆蛋白的有限酶解增强了Caco-2细胞对胆固醇吸收的抑制作用。

Q4 Immunology and Microbiology
May-June Tsou, F. Kao, Jyun-Bi Jhuang, Wen-Dee Chiang
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引用次数: 7

摘要

用风味酶、碱性蛋白酶、胰蛋白酶、凝乳胰蛋白酶和胃蛋白酶水解分离的大豆蛋白(ISP) 12 h,得到蛋白质水解产物。测定每种水解产物的回收率,并评估其在Caco-2细胞中的相对胆固醇摄取(RCU, %)。回收率越高,表示产量越高。RCU越低,对胆固醇吸收的抑制作用越强。结果表明,完整的ISP可以通过水解这些酶进一步降低其RCU。风味酶- isp水解液的RCU最低,为37%,回收率最高,为58%。还研究了风味酶有限水解ISP(水解度DH, %)对Caco-2细胞回收率和RCU的影响。当DH < 22.8%时,RCU随DH的增大而减小。然而,使用风味酶对ISP进行大量水解,得到DH大于28%,并不能保证增强对胆固醇吸收的抑制,反而显著降低了回收率(p<0.05)。通过透射电镜对胶束进行检查,发现水解产物明显融入胶束膜,而ISP则没有。这一发现表明,在胶束制备过程中,水解液的掺入会降低胆固醇的溶解度,并抑制Caco-2细胞对胆固醇的吸收。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Limited enzymatic hydrolysis of soy protein enhances cholesterol absorption inhibition in Caco-2 cells.
Protein hydrolysates were produced by hydrolyzing isolated soy protein (ISP) with enzymes including flavourzyme, alcalase, trypsin, chymotrypsin and pepsin for 12 h, respectively. Each hydrolysate was measured its recovery and evaluated its relative cholesterol uptake (RCU, %) in Caco-2 cells. The higher the recovery represented the higher the yield. The lower the RCU revealed the higher the cholesterol absorption inhibition. The result indicated that intact ISP could further decrease its RCU by hydrolyzing with any of these enzymes. Flavourzyme-ISP hydrolysate had the lowest RCU of 37% and maintained the highest recovery of 58% than the others. The effects of limited ISP hydrolysis using flavourzyme in terms of the degree of hydrolysis (DH, %) on recovery and RCU in Caco-2 cells were also investigated. When the DH was less than 22.8%, the RCU decreased with the increase in DH. However, extensive ISP hydrolysis using flavourzyme to obtain DH higher than 28% did not guarantee enhance the cholesterol absorption inhibition but significantly (p<0.05) decreased the recovery. According to micelle inspection using a transmission electron microscope, the hydrolysate was clearly incorporated into the micellar membrane but ISP wasn't. This finding suggested that incorporation of the hydrolysate lead to reduce cholesterol solubility during micelle preparation as well as inhibit cholesterol absorption in Caco-2 cells.
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来源期刊
Taiwanese Journal of Agricultural Chemistry and Food Science
Taiwanese Journal of Agricultural Chemistry and Food Science Immunology and Microbiology-Applied Microbiology and Biotechnology
CiteScore
0.10
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