蓖麻油的药理作用及其苦含量体外抗疟活性评价

Muyumba Nsomue Joachim, Bolangongo Mpandu Naomie, Mulula Arnold, Mbuyi Katshiatshia Haddy, K. T. Kashishi, T. Kalulu, Joséphine K. Ntumba
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引用次数: 2

摘要

目的:提取蓖麻油的苦含量,并评价其苦含量的抗疟活性。研究设计:实验研究。学习地点和时间:2016年12月至2018年6月,金沙萨大学化学系和国家生物医学研究所。方法:在刚果民主共和国的Kwilu地区采集了procera的种子。采用标准化索氏法提取并测定其含油量。采用液-液萃取法去除原仁油中的苦味成分。感官分析已经使用了一种去苦味有效性的测试。采用滋养体分裂体成熟试验法,对苦提取物、提取物、粗提物和脱臭油对恶性疟原虫的体外抗疟活性进行了评价。结果:测定了原油和脱脂油的酸值(12.72和2.72 mg KOH/g)、皂化值(190.36和193.12 mg KOH/g)、过氧化值(5.26和19.66 meq O2/Kg)和碘值(69.09和68.63 g I2/100 g)等质量指标,并对其抗疟活性进行了评价。粗提油和苦参的抗疟活性分别为3.44和0.30µg/mL。结论:去苦不影响果仁油的质量。这些结果在很大程度上符合食用油食品法典的规定。在抗疟活性方面,根据RITAM测定,原麻粗油对恶性疟原虫的抗疟活性可能为3.44 μg/mL,低于苦味含量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Valorization of Carapa procera Oil and Evaluation in vitro of Antimalarial Activity of Its Bitter Content
Aims: To extract the bitter content of Carapa procera oil and to evaluate the antimalarial activity of its bitter content. Study Design:  Experimental research. Place and Duration of Study: Department of Chemistry (University of Kinshasa) and National Institute for Biomedical Research, between December 2016 and June 2018. Methodology: The seeds of C. procera were collected in the Kwilu region (Democratic Republic of Congo). A standardized method of Soxhlet was used for the extraction and determination of the oil content. The bitter content of C. procera oil was removed by liquid-liquid extraction. Organoleptic sensory analyzes have used a test of the effectiveness of debittering. The in vitro antimalarial activity of bitter content, extracts, crude oil and the debittered oil on Plasmodium falciparum were evaluated according to the method of maturation test of trophozoites into schizonts. Results: Quality indexes such as acid values (12.72 and 2.72 mg KOH/g), saponification values (190.36 and 193.12 mg KOH/g), peroxide values (5.26 and 19.66 meq O2/Kg), and iodine values (69.09 and 68.63 g I2/100 g) was determined for crude oil and debittered oil respective antimalarial activity was evaluated. Crude oil and bitter content showed respectively significant antimalarial activity with IC50 values of 3.44 and 0.30 µg/mL. Conclusion: The elimination of the bitter content does not affect the quality of C. procera oil. These results were, for the most part, in accordance with the codex alimentarius for edible oils. Concerning the antimalarial activity, according to RITAM, C. procera crude oil could present probable antimalarial activity against P. falciparum (3.44 μg/mL), low value compared to that of the bitter content.
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