黑虾微流控细胞分离及遗传分析

2021 21st International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers) Pub Date : 2021-06-20 DOI:10.1109/Transducers50396.2021.9495666

Tomoki Murakami, Hiroaki Suzuki, K. Koiwai

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引用次数: 0

摘要

我们利用微流体技术，利用确定性横向位移(DLD)方法，根据细胞大小从非模式生物中分离出免疫细胞。在本研究中，我们以虾(Marsupenaeus japonicus)作为一种在世界范围内广泛养殖的非模式生物为研究对象。我们用流式细胞仪(FCM)和图像分析评估了靶细胞的大小分离效率。此外，为了预测分离细胞的功能，我们对分离细胞进行了qRT-PCR基因表达分析。结果表明，不同大小的细胞具有不同的免疫功能。

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Microfluidic Cell Separation and Genetic Analysis of Kuruma Shrimp

We used microfluidics to separate immune cells from non-model organisms based on the cell size using the Deterministic Lateral Displacement (DLD) method. In this study, we targeted shrimp (Marsupenaeus japonicus) as a non-model organism that is widely cultured all over the world. We evaluated the efficiency of the size separation of target cells by flow cytometer (FCM) and image analysis. Besides, to predict the functions of the separated cells, we conducted gene expression analysis on the separated cells by qRT-PCR. The results showed that different sizes of cells have different immune functions.

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2021 21st International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers)

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