On the efficacy of dielectric spectroscopy in the identification of onset of the various stages in lactic acid coagulation of milk

Abstract There are five stages in acid coagulation of milk and most studies miss out on a stage or two depending on the experiments performed. Dielectric studies of milk and various physico-chemical studies on the formation of yogurt are well studied and established. However, most of the papers deal with either a limited range of frequencies in the dielectric studies and hence are unable to reflect all the mechanisms of coagulation that occur, or are done as stand-alone experiments, with no correlations to other physico-chemical processes. In this paper, we discuss the broadband dielectric spectroscopy of lactic-acid coagulation of milk, identify the various stages, and compare it with pH, zeta potential, particle size and microscopy. We establish the superiority of dielectric spectroscopy in the identification of all stages of coagulation, the indifference of the process to fat content. Further a strong correlation between tan δ and pH is seen, that is an indicator of the five different stages of lactic-acid induced coagulation. Penetration depth is calculated as milk coagulates and it is seen to be decreasing with coagulation. Broadband dielectric spectroscopy is seen to reveal the onset of coagulation much earlier than other experimental techniques discussed here.