Channel disassembled: Pick, tweak, and soak parts to soften

Mechanosensitive channels are the molecules closely matching the definition of “machines” in our macroworld—they convert external mechanical forces into motion of the parts to open or close a water-filled pore, with no chemical energy inputs involved. Bacterial mechanosensitive channel MscL from E. coli is the first known and probably the best understood representative of this group. A minimalistic bundle of 5 pairs of interlocked a-helices in the membrane requires no external protein connections. The barrel responds directly to the lipid bilayer tension by tilting of the helices and iris-like expansion that opens a large (»3 nm) pore. Robustness of MscL makes it a promising template for engineering nano-devices operated by force or chemical modification. Known crystal structures and models enabled molecular simulations which visualized lipid interactions and forces acting on specific channel segments. The experimentally estimated timescale of the opening, is in the order of microsecond, whereas the process of subsequent equilibration within the subset of open states may take up to several seconds. Simulations striving for proper representation of these stochastic events must be at least as long. Atomistic simulations on this time scale are currently unattainable thus calling for radical simplifications. Over the last decade, a helpful approximation for MscL was developed using solid-state mechanical engineering tools that present the channel/membrane system as interacting meshworks of finite elastic elements. Despite the apparent simplicity, MscL is surrounded by solvent and a highly anisotropic bilayer that both change their interactions with the protein during the gating. This poses several challenges for the finiteelement approach. One is the necessity to account for large changes of the solvent-accessible area and associated hydration contribution. In part, it was recently addressed by explicitly introducing terms for the hydration energetics. The second oversimplification of the finite-element approach was that, while effective elasticities of different elements can be derived from atomistic calculations, they were assumed to be constant and it is unclear how they change under varying solvation or sequestration inside the lipid. The paper by Bavi et al. published in the current issue addresses these questions. Their elasticity tests of isolated a-helices of MscL in steered molecular dynamics simulations gave different moduli estimations for different domains. As one can expect, the elastic modulus values changed to some extent upon switching from M. tuberculosis MscL to its modeled E. coli homolog. Most importantly, the a-helices became considerably softer when the helix was hydrated, compared with simulations in vacuo. That finding might be crucial for the mechanics of MscL in continuum-based models because the hydration of the pore-lining helices increases dramatically upon channel opening. Moreover, as the lipid-facing helices tilt in the stretching (and thinning) bilayer, their hydration is likely to change too. This calls for advancement of the finite-element model to the one where elements are sensitive to the changing environment and adjust their stiffness through the simulation. The change in elasticity can be essential for the gating time course and may potentially contribute to the well-