人富血小板血浆和l -抗坏血酸对人脂肪干细胞形态、增殖和成软骨能力的影响

I. Rosadi, K. Karina, I. Rosliana, S. Sobariah, I. Afini, Tias Widyastuti, A. Barlian
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引用次数: 16

摘要

背景:小耳是一种由软骨缺损引起的先天性外耳畸形。脂肪源性干细胞(ADSC)是一种很有前途的用于小体软骨组织工程的细胞。在这项研究中,我们重点研究了ADSC在三种不同培养基中的增殖和软骨形成,这三种培养基由10%胎牛血清(FBS)、10%胎牛血清加l -抗坏血酸和10%富血小板血浆(PRP)组成。方法:诱导ADSC分化为脂肪细胞、软骨细胞和骨细胞。比较了三种培养基中ADSC的形态、增殖和倍增时间。每7天进行一次观察和阿利新蓝染色,以评估每次治疗后ADSC的软骨形成能力。结果:分离的ADSC能够分化为脂肪细胞、骨细胞和软骨细胞。各组ADSC均呈成纤维细胞样形态,但10%胎牛血清和10%胎牛血清加LAA组ADSC细胞变扁平且变大。10% PRP组ADSC增殖快于10% FBS,无论是否有LAA。10% PRP组、10% FBS加LAA组和10% FBS组ADSC的PDT值分别为34小时、44小时和48小时。阿利新蓝染色显示,添加10% LAA和10% PRP的10%胎牛血清中的ADSC在培养时间延长至21天后能够进行软骨形成,而10%胎牛血清中的ADSC则无法进行软骨形成。结论:在培养基中添加10%的LAA和10%的PRP可促进ADSC的增殖和软骨形成。关键词:人ADSC, PRP, l -抗坏血酸,增殖,软骨形成
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Effect of Human Platelet-Rich Plasma and L-Ascorbic Acid on Morphology, Proliferation, and Chondrogenesis Ability towards Human Adipose-Derived Stem Cells
Background: Microtia is a congenital malformation in the external ear due to cartilage defect. Adipose-derived stem cells (ADSC) is promising cells to develop cartilage tissue engineering for microtia. In this study, we focused on proliferation and chondrogenesis of ADSC in three different media, which consist of 10% fetal bovine serum (FBS), 10% FBS with L-ascorbic acid, and 10% human platelet rich plasma (PRP). Methods: ADSC were induced to differentiate into adipocytes, chondrocyte and osteocytes. ADSC morphology, proliferation and population doubling time was compared in three different media and analysed. Observation and alcian blue staining were done every 7 days to assess chondrogenic potency of ADSC from each treatment.Results: Isolated ADSC were able to differentiate into adipocytes, osteocytes and chondrocytes. ADSC in all group have fibroblast-like morphology, but cells in 10% FBS and 10% FBS with LAA group were flattened and larger. ADSC in 10% PRP group proliferates faster than 10% FBS with and without LAA. PDT values of ADSC were 34 hours, 44 hours and 48 hours, respectively for 10% PRP, 10% FBS with LAA and 10% FBS group. Alcian blue staining revealed that ADSC in 10% FBS with LAA and 10% PRP were able to proceed to chondrogenesis when cultured time were prolong up to 21 days, but not with ADSC in 10% FBS. Conclusion: We conclude that adding 10% FBS with LAA or 10% PRP into medium culture can support proliferation and chondrogenesis of ADSC. Key words: human ADSC, PRP, L-ascorbic acid, proliferation, chondrogenesis
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