Castilla (Solanum quitoense Lam.)多酚氧化酶基因的分子特征

D. G. Arias, C. Doria, L. R. Ramos, Heydy Catalina Navia Morocho
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引用次数: 5

摘要

露露(Solanum quitoense Lam.)是一种来自安第斯山脉的异国水果,具有很高的出口潜力。然而,采收期间和采收后过程中产生的褐变改变了这种水果的感官和营养特性,这使得其作为新鲜水果进行管理变得困难。褐变过程是由位于叶绿体类囊体中的多酚氧化酶(PPOs)家族调控的。当组织水平受到损伤时,在液泡中发现的酚类化合物与多酚氧化酶接触。这种酶在酚类化合物之间,以及它们与蛋白质和细胞壁之间产生聚合。本研究基于DNA和RNA样本,在遗传水平上分析了卡斯蒂亚仙人掌的多酚氧化酶。结果表明,该酶与其他植物多酚氧化酶具有较高的同源性。与龙葵(Solanum melongena L.)同源性最高,属细茎属(Leptostemonum)。鉴定了多酚氧化酶特有的酪氨酸酶和两个铜结合结构域、维持自然环境的保守残基、针对叶绿体的信号肽序列以及用于识别小分子的转录调控的UTRA结构域。使用Southern blot分析基因拷贝数,在lulo基因组中确定了至少8个基因拷贝。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Molecular characterization of the polyphenol oxidase gene in lulo (Solanum quitoense Lam.) var. Castilla
Lulo (Solanum quitoense Lam.) is an exotic fruit from the Andes Mountains with a high export potential. However, the browning that is produced during harvest and the postharvest processes alters the organoleptic and nutritional properties of this fruit, which has made its management as a fresh fruit difficult. The browning processes are regulated by the enzyme family of the polyphenol oxidases (PPOs) located in the thylakoids of the chloroplast. When there is damage at the tissue level, the phenolic compounds found in the vacuoles enter into contact with the polyphenol oxidase. This enzyme produces polymerization among the phenolic compounds, as well as between them and the proteins and cell walls. This study analyzed the polyphenol oxidase in lulo var. Castilla at the genetic level, based on DNA and RNA samples. The results showed a high level of homology with other polyphenol oxidases from plants. The highest degree of homology was found with Solanum melongena L., which belongs to the same clade, Leptostemonum. The tyrosinase and two copper-binding domains, characteristic of the polyphenol oxidase, the conserved residues that maintain the natural environment, the sequence of a signal peptide for targeting chloroplast, and the UTRA domain of transcription regulation for recognizing small molecules were identified. Southern blot was used to analyze the number of gene copies, identifying at least eight ones in the lulo genome.
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