An Attempt to Study of Mouse ES Cell Differentiation using Collagen Derived from Tilapia Scale

89 INTRODUCTION Three-dimensional culture is required to regenerate tissues and organs of interest through cell differentiation. Many three-dimensional culture techniques utilize a cell mixture in a scaffold . Porcine collagen has traditionally been used as a scaffold and put to practical use in skin 1, 4, 5 and mucosal models . We have conducted various studies on ES cell differentiation using porcine atelocollagen. However, even if tissues and organs can be generated in three-dimensional culture in collagen derived from mammals, such as pigs, there remain problems, such as BSE and viruses. Fish are considered to be free of these problems, because humans and pigs are mammals, while fish are species distinct from mammals. Extraction Technology of Collagen from tilapia scales have been developed in 2003 . We previously cultured mouse ES cells using collagen derived from An Attempt to Study of Mouse ES Cell Differentiation using Collagen Derived from Tilapia Scale