Biodentine stimulates the migratory and biological responses of human gingival fibroblast

Introduction: Biodentine (BD), a dentin substitute, is currently used to treat external cervical root resorption, but its effects on gingival fibroblasts (GFs) are not fully known. Objective: To investigate and compare BD and MTA (mineral trioxide aggre-gate) in terms of proliferative, migratory, and adhesion effects on human pulpal and gingival cells. Material and Methods: Cells were incubated directly on the surface of BD and MTA disks. Adhesion (4 and 24 h) and proliferation (3, 5, 7, 14, 21) were evaluated with crystal violet and MTT assays (n=9 X each group). A wound-healing assay was performed for cell migration, with 0.2 and 2 µg/ml MTA or BD (n=6 X each group). The cut-off point for statistical significance was set at p<0.05, p<0.01 and p<0.001. Results: The best adhesion and proliferation results for gingival fibroblast (GFs) were obtained with BD (p<0.01). MTA and BD enhanced the migration of GFs in a dose-dependent manner, with superior results with BD, and 2 µg/ml was the optimal concentration for enhancing the migration of GFs. Conclusion: Results indicate that BD and MTA exhibit excellent compatibility in terms of cell adhesion, proliferation, and cellular migration. Also, the results suggested that BD is associated with better results than MTA in GFs. The results support the clinical application of BD in areas colonized with GFs.