Medaka Extended One-Generation Reproduction Test (MEOGRT) (OECD TG 240)

468. Modality detected/endpoints: This fish life cycle test was specifically designed to investigate the apical effects of endocrine disrupters, and has several endpoints which can be considered diagnostic of some types of estrogen/androgen/thyroid/steroidogenesis (E,A,T,S) activity. This gives it an advantage over other currently standardised life cycle tests, and its use for evaluating endocrine disruptors (EDs) is to be preferred to the Fish Life Cycle Toxicity Test (see Section C.2.21) which, although sensitive to the apical effects of some EDs, contains no endocrine-sensitive endpoints. In view of the inclusion of certain ED-specific endpoints, the MEOGRT can contribute useful evidence about the probable causality of apical effects, which is a key issue in the definition of EDs.