Electroporation Improvement of Leukemic Cells Using Dielectrophoresis Technique

Electroporation is a powerful technique used to improve the permeability of the cell membrane; pores are created in the cell membrane based on the application of a high voltage. The high voltage improves the number of membrane pores, however, it might kill the cells. Electroporation is achieved using a microelectrode placed in a microfluidic device. This paper focuses on the improvement of the electroporation of a cell based on levitation dielectrophoresis and an electrorotation force. Then, the change of the conductivity is detected at both the frequency and time domain, i.e., individual cells are trapped due to the effect of levitation and electrorotation force, and then the variation in the conductivity is detected. We use frequency and time domain spectroscopy to measure the conductivity of the white blood cell, i.e., B-cell, T-cell and leukemia K365. In this paper, we review studies of conductivity separation in a microfluidic device based on a 2-D electrode system. Also, a comparison between the conductivity of leukemia k365, B-cell, and T-cell due to the drag and electrorotaional forces are presented and discussed.