当使用电子烟时,非特异性蛋白水解在肺组织变化发展中的作用

Q4 Medicine
L. L. Aliyev, M. Aliyev, P. E. Maximova, T. Makalish, A. Kubyshkin, E. Zyablitskaya, A. V. Grigoryants, Ya. Karyophyllidis, D. I. Mitrushkin
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引用次数: 0

摘要

近年来,世界各地的电子烟用户数量一直在稳步增长。ES加热并汽化丙二醇、植物甘油、尼古丁和香精混合物的成分。使用ES引起的严重肺损伤的报告,其中一些已导致死亡,迫切需要研究此类并发症的发病机制。其中一种机制是激活非特异性蛋白水解,同时破坏血气屏障的结缔组织成分。研究的目的。研究蛋白酶抑制剂系统指标的动态变化及其在肺组织形态学变化中的作用,采用实验方法模拟es的影响。实验采用24只体重160 ~ 180 g的雄性Wistar大鼠。使用连接到双通道压缩机的POD系统,通过间歇性暴露于烟雾混合物蒸汽来模拟ES的使用。大鼠支气管肺泡灌洗液和血清中非特异性蛋白酶抑制系统的状态分析及形态学研究结果表明,胰蛋白酶样蛋白酶的激活和局部抗胰蛋白酶电位的耗竭导致肺组织的生化变化和随后的结构变化。后者以充血、白细胞浸润和间质增生为特征。此外,暴露于含尼古丁的混合物已被证明会影响蛋白酶抑制系统和血清成分,这与抗胰蛋白酶活性的代偿性增加有关。本研究结果表明,支气管肺泡灌洗液中胰蛋白酶样蛋白酶的激活对肺组织具有损伤作用,导致组织血流动力学紊乱,白细胞外渗,血气屏障重塑和增厚。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The role of nonspecific proteolysis in the development of alterative changes in lung tissue when using electronic cigarettes
In recent years, the number of electronic cigarettes (ES) users has been growing steadily around the world. ES heat and vaporize components of a mixture of propylene glycol, vegetable glycerin, nicotine, and flavors. Reports of severe forms of lung injury caused by the use of ES, some of which have resulted in death, urge studying the pathogenetic mechanisms of such complications. One of the mechanisms is the activation of nonspecific proteolysis accompanied by the destruction of connective tissue components of the blood-air barrier.Aim of the study. To study the dynamics of the proteinase-inhibitor system indicators and its role in the morphological changes of lung tissue in experimental modeling of the effects of ES.Methods. Experimental studies were performed in 24 white male Wistar rats weighing 160 – 180 g. ES use of was simulated by intermittent exposure to smoke mixture vapors using a POD system connected to a two-channel compressor.Results. Analysis of the state of the nonspecific proteinase inhibitory system in rat broncho-alveolar lavage and blood serum and the results of morphological studies indicate activation of trypsin-like proteinases and depletion of the local antitryptic potential, leading to biochemical changes with subsequent structural changes in lung tissue. The latter are characterized by congestion, leukocyte infiltration, and stromal proliferation. In addition, exposure to nicotine-containing mixtures has been shown to affect the proteinase inhibitory system and blood serum composition, which is associated with a compensatory increase in antitrypsin activity.Conclusion. The results of the study indicate the activation of trypsin-like proteinases in broncho-alveolar lavage, which have a damaging effect on lung tissue, leading to disturbances in tissue hemodynamics, extravasation of leukocytes, remodeling and thickening of the blood-air barrier.
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来源期刊
Pulmonologiya
Pulmonologiya Medicine-Pulmonary and Respiratory Medicine
CiteScore
1.40
自引率
0.00%
发文量
70
期刊介绍: The aim of this journal is to state a scientific position of the Russian Respiratory Society (RRS) on diagnosis and treatment of respiratory diseases based on recent evidence-based clinical trial publications and international consensuses. The most important tasks of the journal are: -improvement proficiency qualifications of respiratory specialists; -education in pulmonology; -prompt publication of original studies on diagnosis and treatment of respiratory diseases; -sharing clinical experience and information about pulmonology service organization in different regions of Russia; -information on current protocols, standards and recommendations of international respiratory societies; -discussion and consequent publication Russian consensus documents and announcement of RRS activities; -publication and comments of regulatory documents of Russian Ministry of Health; -historical review of Russian pulmonology development. The scientific concept of the journal includes publication of current evidence-based studies on respiratory medicine and their discussion with the participation of Russian and foreign experts and development of national consensus documents on respiratory medicine. Russian and foreign respiratory specialists including pneumologists, TB specialists, thoracic surgeons, allergists, clinical immunologists, pediatricians, oncologists, physiologists, and therapeutists are invited to publish article in the journal.
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