{"title":"用气相色谱法分析细胞代谢产物和发酵气体","authors":"P. Unger, Z. Vozňáková, J. Páca","doi":"10.1002/JBT.2570270122","DOIUrl":null,"url":null,"abstract":"A method for the estimation of ethanol and volatile fatty acids by gas chromatography by direct treatment of cultivation broth is described. The packing material was Hallcomid M-18 with 1% H3PO4 on Chromosorb W AW. The separation was made at 132°C with nitrogen as a carrier gas; for detection a flame ionisation detector was used. The analysis of oxygen and carbon dioxide of the inlet and outlet gas from a fermentor was also studied. For the separation of the gases the combination of molecular seive 5A and Chromosorb 102 was used. The analysis was performed at 35°C with helium as a carrier gas and a thermal conductivity detector was used. With the experimental conditions found in this study the methods described gave reproducible and accurate results for the analytical control of cell cultivation processes.","PeriodicalId":15255,"journal":{"name":"Journal of biochemical toxicology","volume":"27 1","pages":"150-154"},"PeriodicalIF":0.0000,"publicationDate":"2007-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"13","resultStr":"{\"title\":\"Analysis of cell metabolic products and fermentation gases by gas chromatography\",\"authors\":\"P. Unger, Z. Vozňáková, J. Páca\",\"doi\":\"10.1002/JBT.2570270122\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A method for the estimation of ethanol and volatile fatty acids by gas chromatography by direct treatment of cultivation broth is described. The packing material was Hallcomid M-18 with 1% H3PO4 on Chromosorb W AW. The separation was made at 132°C with nitrogen as a carrier gas; for detection a flame ionisation detector was used. The analysis of oxygen and carbon dioxide of the inlet and outlet gas from a fermentor was also studied. For the separation of the gases the combination of molecular seive 5A and Chromosorb 102 was used. The analysis was performed at 35°C with helium as a carrier gas and a thermal conductivity detector was used. With the experimental conditions found in this study the methods described gave reproducible and accurate results for the analytical control of cell cultivation processes.\",\"PeriodicalId\":15255,\"journal\":{\"name\":\"Journal of biochemical toxicology\",\"volume\":\"27 1\",\"pages\":\"150-154\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2007-05-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"13\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of biochemical toxicology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1002/JBT.2570270122\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of biochemical toxicology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/JBT.2570270122","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 13
摘要
介绍了用气相色谱法直接处理培养液测定乙醇和挥发性脂肪酸的方法。包装材料为Hallcomid M-18，在Chromosorb W W上添加1% H3PO4。以氮气为载气，在132℃下进行分离;为了检测，使用了火焰电离检测器。本文还对发酵罐进出口气体中的氧气和二氧化碳进行了分析。采用5A分子筛和Chromosorb 102相结合的方法进行气体分离。分析在35°C下进行，以氦气为载气，使用导热检测器。在本研究中发现的实验条件下，所描述的方法为细胞培养过程的分析控制提供了可重复性和准确的结果。
Analysis of cell metabolic products and fermentation gases by gas chromatography
A method for the estimation of ethanol and volatile fatty acids by gas chromatography by direct treatment of cultivation broth is described. The packing material was Hallcomid M-18 with 1% H3PO4 on Chromosorb W AW. The separation was made at 132°C with nitrogen as a carrier gas; for detection a flame ionisation detector was used. The analysis of oxygen and carbon dioxide of the inlet and outlet gas from a fermentor was also studied. For the separation of the gases the combination of molecular seive 5A and Chromosorb 102 was used. The analysis was performed at 35°C with helium as a carrier gas and a thermal conductivity detector was used. With the experimental conditions found in this study the methods described gave reproducible and accurate results for the analytical control of cell cultivation processes.
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