J. Gómez, C. Ramo, Juan A. Canales, I. García, M. Castro, Alejandro Pérez-Hurtado, J. Amat
{"title":"两种分析方法提取禽蛋壳色素的比较研究","authors":"J. Gómez, C. Ramo, Juan A. Canales, I. García, M. Castro, Alejandro Pérez-Hurtado, J. Amat","doi":"10.13157/arla.67.1.2020.sc3","DOIUrl":null,"url":null,"abstract":"There are two main methods to extract pigments from avian eggshells. There are those using strong acids (e.g. sulphuric, hydrochloric or formic), that allow for a quantification of pigment concentrations but with which samples are destroyed. Alternatively, there are methods employing weaker acids (e.g. acetic), with which samples are not destroyed, although pigment concentrations may not be quantified and only their presence is detected. In some cases, there may be a need to quantify pigments in samples in which the pigments have already been detected. Here, we assess whether the quantity of pigments extracted from eggshells using a strong acid is affected by previous treatment of eggshells with a weak acid. For this, we used eggshells of Kentish Plovers Charadrius alexandrinus and domestic Japanese Quails Coturnix japonica, and aliquots of individual eggshells treated either with both acids (first applying a weak acid and then a strong acid) or only with the strong acid. Quantities of extracted protoporphyrin and biliverdin (the main eggshell pigments) were lower when using a strong acid after application of a weak acid. However, there were significant positive correlations between the quantities of pigments extracted using the two methods, suggesting that eggshell samples used to determine pigment presence may still be usable to quantify pigment concentrations using a strong acid.—Gomez, J., Ramo, C., Canales, J.A., Garcia, I.M., Castro, M., Perez-Hurtado, A. & Amat, J.A. (2020). A comparative assessment of pigment extraction from avian eggshells using two analytical protocols.","PeriodicalId":55571,"journal":{"name":"Ardeola-International Journal of Ornithology","volume":"50 1","pages":"85-92"},"PeriodicalIF":1.4000,"publicationDate":"2019-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A Comparative Assessment of Pigment Extraction from Avian Eggshells Using Two Analytical Protocols\",\"authors\":\"J. Gómez, C. Ramo, Juan A. Canales, I. García, M. Castro, Alejandro Pérez-Hurtado, J. Amat\",\"doi\":\"10.13157/arla.67.1.2020.sc3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"There are two main methods to extract pigments from avian eggshells. There are those using strong acids (e.g. sulphuric, hydrochloric or formic), that allow for a quantification of pigment concentrations but with which samples are destroyed. Alternatively, there are methods employing weaker acids (e.g. acetic), with which samples are not destroyed, although pigment concentrations may not be quantified and only their presence is detected. In some cases, there may be a need to quantify pigments in samples in which the pigments have already been detected. Here, we assess whether the quantity of pigments extracted from eggshells using a strong acid is affected by previous treatment of eggshells with a weak acid. For this, we used eggshells of Kentish Plovers Charadrius alexandrinus and domestic Japanese Quails Coturnix japonica, and aliquots of individual eggshells treated either with both acids (first applying a weak acid and then a strong acid) or only with the strong acid. Quantities of extracted protoporphyrin and biliverdin (the main eggshell pigments) were lower when using a strong acid after application of a weak acid. However, there were significant positive correlations between the quantities of pigments extracted using the two methods, suggesting that eggshell samples used to determine pigment presence may still be usable to quantify pigment concentrations using a strong acid.—Gomez, J., Ramo, C., Canales, J.A., Garcia, I.M., Castro, M., Perez-Hurtado, A. & Amat, J.A. (2020). 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A Comparative Assessment of Pigment Extraction from Avian Eggshells Using Two Analytical Protocols
There are two main methods to extract pigments from avian eggshells. There are those using strong acids (e.g. sulphuric, hydrochloric or formic), that allow for a quantification of pigment concentrations but with which samples are destroyed. Alternatively, there are methods employing weaker acids (e.g. acetic), with which samples are not destroyed, although pigment concentrations may not be quantified and only their presence is detected. In some cases, there may be a need to quantify pigments in samples in which the pigments have already been detected. Here, we assess whether the quantity of pigments extracted from eggshells using a strong acid is affected by previous treatment of eggshells with a weak acid. For this, we used eggshells of Kentish Plovers Charadrius alexandrinus and domestic Japanese Quails Coturnix japonica, and aliquots of individual eggshells treated either with both acids (first applying a weak acid and then a strong acid) or only with the strong acid. Quantities of extracted protoporphyrin and biliverdin (the main eggshell pigments) were lower when using a strong acid after application of a weak acid. However, there were significant positive correlations between the quantities of pigments extracted using the two methods, suggesting that eggshell samples used to determine pigment presence may still be usable to quantify pigment concentrations using a strong acid.—Gomez, J., Ramo, C., Canales, J.A., Garcia, I.M., Castro, M., Perez-Hurtado, A. & Amat, J.A. (2020). A comparative assessment of pigment extraction from avian eggshells using two analytical protocols.
期刊介绍:
Ardeola: International Journal of Ornithology is the scientific journal of SEO/BirdLife, the Spanish Ornithological Society. The journal had a regional focus when it was first published, in 1954. Since then, and particular during the past two decades, the journal has expanded its thematic and geographical scope. It is now a fully international forum for research on all aspects of ornithology. We thus welcome studies within the fields of basic biology, ecology, behaviour, conservation and biogeography, especially those arising from hypothesis-based research. Although we have a long publication history of Mediterranean and Neotropical studies, we accept papers on investigations worldwide.
Each volume of Ardeola has two parts, published annually in January and July. The main body of each issue comprises full-length original articles (Papersand Review articles) and shorter notes on methodology or stimulating findings (Short Communications). The publication language is English, with summaries, figure legends and table captions also in Spanish. Ardeolaalso publishes critical Book Reviewsand PhD-Dissertation Summaries; summarising ornithological theses defended in Spain. Finally there are two Spanish-language sections, Ornithological News; summarising significant recent observations of birds in Spain, and Observations of Rare Birds in Spain, the annual reports of the Spanish Rarities Committee.