Development and Validation of Stability Indicating Analytical Method for the Simultaneous Estimation of Netarsudil and Latanoprost by RP-HPLC

A simple, Accurate, Precise method was developed for the simultaneous estimation of the Netarsudil and Latanoprost in opthalmic solution dosage form. Chromatogram was run through Zodiacsil C18 150 x 4.6 mm, 5μ. Mobile phase containing 0.01N Ammonium acetate: Methanol taken with in the ratio 55:45 was pumped through column on a flow rate at 1.0ml/min. Buffer used in this method was Ammonium acetate. Temperature was maintained at 30C. Optimized wavelength selected was 225nm. Retention time of Netarsudil and Latanoprost were found to be 2.222 min and 2.706 min. %RSD of the Netarsudil and Latanoprost were and found to be 0.5 and 0.8 respectively. %Recovery was obtained as 100.09% and 100.5% for Netarsudil and Latanoprost respectively. LOD, LOQ values obtained from regression equations of Netarsudil and Latanoprost were 0.07, 0.22 and 0.03, 0.09 respectively. Regression equation of Netarsudil y = 33253x +578.8 and y =45497x + 399.2 of Latanoprost. Retention times were decreased and that runtime was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.