Rita Radzevičiūtė, Anna Budrienė, E. Budrys, Svetlana Orlovskytė, J. Turčinavičienė
{"title":"通过跨种扩增,建立了一组微卫星标记,用于孤巢黄蜂(Ancistrocerus trifasciatus, m<s:1> ller, 1776)","authors":"Rita Radzevičiūtė, Anna Budrienė, E. Budrys, Svetlana Orlovskytė, J. Turčinavičienė","doi":"10.6001/EKOLOGIJA.V58I1.2345","DOIUrl":null,"url":null,"abstract":"Solitary cavity-nesting Hymenoptera constitute a group of important bioindicators of terrestrial habitats. Some of them, like caterpillar-hunting potter wasp Ancistrocerus trifasciatus (Hymenoptera: Vespidae: Eumeninae), are quite abundant in both continuous and fragmented habitats and might be a promising model species for studying the impact of habitat fragmentation and landscape connectivity on genetic diversity of entomophagous insects. Highly polymorphic microsatellites are a powerful molecular tool for intraspecific studies but the development of this marker system de novo is especially laborious and time-consuming. An alternative time- and effort-effective approach to establish the microsatellite markers for the species of interest is the cross-species amplification of loci already isolated in related species. Here we present a panel of five polymorphic microsatellite loci for A. trifasciatus developed by cross-species amplification of twenty-nine microsatellite markers published so far for nearctic potter wasps.","PeriodicalId":35175,"journal":{"name":"Socijalna Ekologija","volume":"29 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2012-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"A panel of microsatellite markers developed for solitary trap-nesting wasp Ancistrocerus trifasciatus (Müller, 1776) by cross-species amplification\",\"authors\":\"Rita Radzevičiūtė, Anna Budrienė, E. Budrys, Svetlana Orlovskytė, J. Turčinavičienė\",\"doi\":\"10.6001/EKOLOGIJA.V58I1.2345\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Solitary cavity-nesting Hymenoptera constitute a group of important bioindicators of terrestrial habitats. Some of them, like caterpillar-hunting potter wasp Ancistrocerus trifasciatus (Hymenoptera: Vespidae: Eumeninae), are quite abundant in both continuous and fragmented habitats and might be a promising model species for studying the impact of habitat fragmentation and landscape connectivity on genetic diversity of entomophagous insects. Highly polymorphic microsatellites are a powerful molecular tool for intraspecific studies but the development of this marker system de novo is especially laborious and time-consuming. An alternative time- and effort-effective approach to establish the microsatellite markers for the species of interest is the cross-species amplification of loci already isolated in related species. Here we present a panel of five polymorphic microsatellite loci for A. trifasciatus developed by cross-species amplification of twenty-nine microsatellite markers published so far for nearctic potter wasps.\",\"PeriodicalId\":35175,\"journal\":{\"name\":\"Socijalna Ekologija\",\"volume\":\"29 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2012-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Socijalna Ekologija\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.6001/EKOLOGIJA.V58I1.2345\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Social Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Socijalna Ekologija","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.6001/EKOLOGIJA.V58I1.2345","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Social Sciences","Score":null,"Total":0}
A panel of microsatellite markers developed for solitary trap-nesting wasp Ancistrocerus trifasciatus (Müller, 1776) by cross-species amplification
Solitary cavity-nesting Hymenoptera constitute a group of important bioindicators of terrestrial habitats. Some of them, like caterpillar-hunting potter wasp Ancistrocerus trifasciatus (Hymenoptera: Vespidae: Eumeninae), are quite abundant in both continuous and fragmented habitats and might be a promising model species for studying the impact of habitat fragmentation and landscape connectivity on genetic diversity of entomophagous insects. Highly polymorphic microsatellites are a powerful molecular tool for intraspecific studies but the development of this marker system de novo is especially laborious and time-consuming. An alternative time- and effort-effective approach to establish the microsatellite markers for the species of interest is the cross-species amplification of loci already isolated in related species. Here we present a panel of five polymorphic microsatellite loci for A. trifasciatus developed by cross-species amplification of twenty-nine microsatellite markers published so far for nearctic potter wasps.