{"title":"用同工酶对小麦-美眉附加系进行鉴定","authors":"J.-Ch. Schmidt , V. Schubert , W.-D. Blüthner","doi":"10.1016/S0015-3796(11)80141-1","DOIUrl":null,"url":null,"abstract":"<div><p>Sixteen leaf and three seed isozymes were tested for their ability to identify alien chromosomes in <em>Triticum aestivum</em> — <em>Aegilops markgrafii</em> amphiploid and six descending disomic addition lines. Four leaf isozymes (NADP-dependent aromatic alcohol dehydrogenase, aspartate aminotransferase, esterase, leucine aminopeptidase) and three seed enzymes (alcohol dehydrogenase, (ß-amylase, esterase) from <em>Ae. markgrafii</em> were found to be informative in differentiating both the amphiploid and five of the six different addition lines from wheat. Leaf peroxidase, an eighth informative marker among the 19 isozyme systems tested was only in the amphiploid present, none of the six addition lines showed the <em>Aegilops-specific</em> band.</p></div>","PeriodicalId":8798,"journal":{"name":"Biochemie und Physiologie der Pflanzen","volume":"188 6","pages":"Pages 385-392"},"PeriodicalIF":0.0000,"publicationDate":"1993-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0015-3796(11)80141-1","citationCount":"13","resultStr":"{\"title\":\"Use of Isozymes to Characterize Triticum aestivum — Aegilops markgrafii Addition Lines\",\"authors\":\"J.-Ch. Schmidt , V. Schubert , W.-D. Blüthner\",\"doi\":\"10.1016/S0015-3796(11)80141-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Sixteen leaf and three seed isozymes were tested for their ability to identify alien chromosomes in <em>Triticum aestivum</em> — <em>Aegilops markgrafii</em> amphiploid and six descending disomic addition lines. Four leaf isozymes (NADP-dependent aromatic alcohol dehydrogenase, aspartate aminotransferase, esterase, leucine aminopeptidase) and three seed enzymes (alcohol dehydrogenase, (ß-amylase, esterase) from <em>Ae. markgrafii</em> were found to be informative in differentiating both the amphiploid and five of the six different addition lines from wheat. Leaf peroxidase, an eighth informative marker among the 19 isozyme systems tested was only in the amphiploid present, none of the six addition lines showed the <em>Aegilops-specific</em> band.</p></div>\",\"PeriodicalId\":8798,\"journal\":{\"name\":\"Biochemie und Physiologie der Pflanzen\",\"volume\":\"188 6\",\"pages\":\"Pages 385-392\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1993-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0015-3796(11)80141-1\",\"citationCount\":\"13\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochemie und Physiologie der Pflanzen\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0015379611801411\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemie und Physiologie der Pflanzen","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0015379611801411","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Use of Isozymes to Characterize Triticum aestivum — Aegilops markgrafii Addition Lines
Sixteen leaf and three seed isozymes were tested for their ability to identify alien chromosomes in Triticum aestivum — Aegilops markgrafii amphiploid and six descending disomic addition lines. Four leaf isozymes (NADP-dependent aromatic alcohol dehydrogenase, aspartate aminotransferase, esterase, leucine aminopeptidase) and three seed enzymes (alcohol dehydrogenase, (ß-amylase, esterase) from Ae. markgrafii were found to be informative in differentiating both the amphiploid and five of the six different addition lines from wheat. Leaf peroxidase, an eighth informative marker among the 19 isozyme systems tested was only in the amphiploid present, none of the six addition lines showed the Aegilops-specific band.
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